Home MAPK ERK inhibitor - NF-κB inhibitor Hypaphorine For research use only.

Hypaphorine

Hypaphorine which is abundantly found in vaccaria semen, counteracts inflammation via inhibition of ERK or/and NFκB signaling pathways.

Hypaphorine Chemical Structure

Hypaphorine Chemical Structure

CAS No. 487-58-1

Purity & Quality Control

Batch: S091101 Water]16 mg/mL]false]DMSO]Insoluble]false]Ethanol]Insoluble]false Purity: 99.99%
99.99

Hypaphorine Related Products

Signaling Pathway

ERK Signaling Pathways

ERK Signaling Pathway

Biological Activity

Description Hypaphorine which is abundantly found in vaccaria semen, counteracts inflammation via inhibition of ERK or/and NFκB signaling pathways.
In vitro
In vitro

Hypaphorine down-regulates LPS-stimulated expressions of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), retards LPS-induced phosphorylation of ERK, nuclear factor kappa beta (NFκB), NFκB inhibitor IκBα, and IKKβ, also eliminates the nuclear translocation of NFκB in LPS-treated RAW 264.7 cells.[1]
Cell Research Cell lines RAW 264.7 cells
Concentrations 6.25, 12.5, 25, 50, 100, 200 μM
Incubation Time 24 h
Method

The cell growth was arrested by incubation of the cells in 2% serum medium for 24 h before treatment. The indicated cells were then seeded in 96-well culture plates at a density of 5×10<sup>3</sup> cells/well, and stimulated with different concentrations of vaccaria hypaphorine (6.25, 12.5, 25, 50, 100, 200 μM) at 37℃ in 5% CO<sub>2</sub> saturated humidity condition for 24 h. Finally, the optical density (OD) was measured at 540 nm.
In Vivo
In vivo

Hypaphorine impairs RANKL-induced osteoclastogenesis through reduction of extracellular signal-regulated kinases (ERK), p38, c-Jun N-terminal kinase (JNK) and NF-κB p65 phosphorylation, which is considered as potential therapeutic agent for treating osteoclast-based bone loss.[2]
Animal Research Animal Models C57BL/6J mice induced by LPS
Dosages 10 and 30 mg/kg
Administration p.o.

Chemical Information & Solubility

Molecular Weight 246.3 Formula

C14H18N2O2
CAS No. 487-58-1 SDF --
Smiles C[N+](C)(C)C(CC1=CNC2=CC=CC=C21)C(=O)[O-]
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

Water : 16 mg/mL

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : Insoluble


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