Hypaphorine

Catalog No.S0911 Batch:S091101

Technical Data

Formula	C₁₄H₁₈N₂O₂
Molecular Weight	246.3	CAS No.	487-58-1
Solubility (25°C)*	In vitro	Water	16 mg/mL (64.96 mM)
		DMSO	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Hypaphorine which is abundantly found in vaccaria semen, counteracts inflammation via inhibition of ERK or/and NFκB signaling pathways.
In vitro	Hypaphorine down-regulates LPS-stimulated expressions of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), retards LPS-induced phosphorylation of ERK, nuclear factor kappa beta (NFκB), NFκB inhibitor IκBα, and IKKβ, also eliminates the nuclear translocation of NFκB in LPS-treated RAW 264.7 cells.^[1]
In vivo	Hypaphorine impairs RANKL-induced osteoclastogenesis through reduction of extracellular signal-regulated kinases (ERK), p38, c-Jun N-terminal kinase (JNK) and NF-κB p65 phosphorylation, which is considered as potential therapeutic agent for treating osteoclast-based bone loss.^[2]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines RAW 264.7 cells Concentrations 6.25, 12.5, 25, 50, 100, 200 μM Incubation Time 24 h Method The cell growth was arrested by incubation of the cells in 2% serum medium for 24 h before treatment. The indicated cells were then seeded in 96-well culture plates at a density of 5×10<sup>3</sup> cells/well, and stimulated with different concentrations of vaccaria hypaphorine (6.25, 12.5, 25, 50, 100, 200 μM) at 37℃ in 5% CO<sub>2</sub> saturated humidity condition for 24 h. Finally, the optical density (OD) was measured at 540 nm.
Animal Study:^{^[2]}	Animal Models C57BL/6J mice induced by LPS Dosages 10 and 30 mg/kg Administration p.o.

Cell Assay:^{^[1]}

Cell lines
RAW 264.7 cells
Concentrations
6.25, 12.5, 25, 50, 100, 200 μM
Incubation Time
24 h
Method
The cell growth was arrested by incubation of the cells in 2% serum medium for 24 h before treatment. The indicated cells were then seeded in 96-well culture plates at a density of 5×10<sup>3</sup> cells/well, and stimulated with different concentrations of vaccaria hypaphorine (6.25, 12.5, 25, 50, 100, 200 μM) at 37℃ in 5% CO<sub>2</sub> saturated humidity condition for 24 h. Finally, the optical density (OD) was measured at 540 nm.

Animal Study:^{^[2]}

Animal Models
C57BL/6J mice induced by LPS
Dosages
10 and 30 mg/kg
Administration
p.o.

References

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.