Home Immunology & Inflammation COX inhibitor (-)-Epicatechin gallate For research use only.

(-)-Epicatechin gallate

Synonyms: (−)-ECG, (−)-Epicatechin 3-gallate, (−)-Epicatechin-3-O-gallate

(-)-Epicatechin gallate (ECG) is an inhibitor of cyclooxygenase-1 (COX-1) with IC50 of 7.5 μM.

(-)-Epicatechin gallate Chemical Structure

(-)-Epicatechin gallate Chemical Structure

CAS No. 1257-08-5

Purity & Quality Control

(-)-Epicatechin gallate Related Products

Signaling Pathway

COX Signaling Pathways

COX Signaling Pathway

Biological Activity

Description (-)-Epicatechin gallate (ECG) is an inhibitor of cyclooxygenase-1 (COX-1) with IC50 of 7.5 μM.
In vitro
In vitro Treatment of HuH7 cells with (–)-Epigallocatechin gallate (EGCG) causes a time- and dose-dependent decrease in the expression of VEGFR-2 and p-VEGFR-2 proteins. EGCG effectively suppresses the growth of HuH7 human HCC cells both in vitro and in vivo[1]. EGCG potently modulates cardiac excitation contraction coupling by acting on RyR and possibly on L-type Ca2+-channel and NaCa exchanger[2].
Cell Research Cell lines HuH7 or Hc cells
Concentrations 0-100 μg/mL
Incubation Time 48 h
Method Three thousand HuH7 or Hc cells are seeded into 96-well plates. Twenty-four hours later, the cells are treated with the indicated concentrations of EGCG (0–100 μg/mL) for 48 h in DF10 medium, and cell viability is examined. All assays are carried out in triplicate.
In Vivo
In vivo Drinking of EGCG significantly inhibits the growth of HuH7 xenografts in nude mice and this is associated with inhibition of the activation of VEGFR-2 and its related downstream signaling molecules, including ERK and Akt. EGCG drinking also decreases the expression of Bcl-xL protein and VEGF mRNA in the xenografts[1].
Animal Research Animal Models BALB/c nude mice (5 weeks of age)
Dosages 0.01 or 0.1%
Administration oral administration

Chemical Information & Solubility

Molecular Weight 442.37 Formula

C22H18O10
CAS No. 1257-08-5 SDF --
Smiles C1C(C(OC2=CC(=CC(=C21)O)O)C3=CC(=C(C=C3)O)O)OC(=O)C4=CC(=C(C(=C4)O)O)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 88 mg/mL ( (198.92 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


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In vivo
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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