(-)-Epicatechin gallate

Catalog No.S3925 Batch:S392501

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Technical Data

Formula

C22H18O10
Molecular Weight 442.37 CAS No. 1257-08-5
Solubility (25°C)* In vitro DMSO 88 mg/mL (198.92 mM)
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description (-)-Epicatechin gallate (ECG) is an inhibitor of cyclooxygenase-1 (COX-1) with IC50 of 7.5 μM.
In vitro Treatment of HuH7 cells with (–)-Epigallocatechin gallate (EGCG) causes a time- and dose-dependent decrease in the expression of VEGFR-2 and p-VEGFR-2 proteins. EGCG effectively suppresses the growth of HuH7 human HCC cells both in vitro and in vivo[1]. EGCG potently modulates cardiac excitation contraction coupling by acting on RyR and possibly on L-type Ca2+-channel and NaCa exchanger[2].
In vivo Drinking of EGCG significantly inhibits the growth of HuH7 xenografts in nude mice and this is associated with inhibition of the activation of VEGFR-2 and its related downstream signaling molecules, including ERK and Akt. EGCG drinking also decreases the expression of Bcl-xL protein and VEGF mRNA in the xenografts[1].

Protocol (from reference)

Cell Assay:[1]
  • Cell lines

    HuH7 or Hc cells

  • Concentrations

    0-100 μg/mL

  • Incubation Time

    48 h

  • Method

    Three thousand HuH7 or Hc cells are seeded into 96-well plates. Twenty-four hours later, the cells are treated with the indicated concentrations of EGCG (0–100 μg/mL) for 48 h in DF10 medium, and cell viability is examined. All assays are carried out in triplicate.

Animal Study:[1]
  • Animal Models

    BALB/c nude mice (5 weeks of age)

  • Dosages

    0.01 or 0.1%

  • Administration

    oral administration

Selleck's (-)-Epicatechin gallate has been cited by 3 publications

Glutamine metabolism regulates FLIP expression and sensitivity to TRAIL in triple-negative breast cancer cells. [ Cell Death Dis, 2018, 9(2):205] PubMed: 29434187
Epigallocatechin-3 gallate prevents pressure overload-induced heart failure by up-regulating SERCA2a via histone acetylation modification in mice. [ PLoS One, 2018, 13(10):e0205123] PubMed: 30286210
EGCG: Potential application as a protective agent against grass carp reovirus in aquaculture. [ J Fish Dis, 2018, 10.1111/jfd.12819] PubMed: 29806139

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.