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DNPH1i(N6-benzyladenosine)

Name: DNPH1i(N6-benzyladenosine)
Brand: Selleck Chemicals
SKU: E1093
Rating: 5 (1 reviews)

Synonyms: N6-benzyladenosine, BAPR

Catalog No.E1093 Purity:99.90%

N6-benzyladenosine (DNPH1i ) is an effective antiproliferative agent both in vitro and in vivo. It is a competitive inhibitor of DNPH1, limiting the activity of DNPH1 in vitro, and a competitive inhibitor of adenosine deaminase (ADA) in L-1210 cells.

DNPH1i(N6-benzyladenosine) Chemical Structure

CAS No. 4294-16-0

Purity & Quality Control

Batch: Purity: 99.90%

99.90

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Biological Activity

Description	N6-benzyladenosine (DNPH1i ) is an effective antiproliferative agent both in vitro and in vivo. It is a competitive inhibitor of DNPH1, limiting the activity of DNPH1 in vitro, and a competitive inhibitor of adenosine deaminase (ADA) in L-1210 cells.

In vitro
In vitro	DNPH1i is the DNPH1 competitive inhibitor, that co-treatmented with hmdU selectively kills the HR-deficient cells, BRCA1-defective cells and PARPi-resistant BRCA1-mutant cell lines depleted of PARP1, sensitizing BRCA-deficient cells to PARPi or hmdU treatment.^[2]
Cell Research	Cell lines	SUM149 BRCA1^mut (parental) and WT (revertant) cell lines
	Concentrations	0.3 µM
	Incubation Time	8 days
	Method	SUM149 BRCA1^mut (parental) and WT (revertant) cell lines are either untreated or treated with hmdU in the absence or presence of DNPH1i (0.3 μM) for 8 days. Viability is determined using CellTiter-Glo.

References

Chemical Information & Solubility

Molecular Weight	357.36	Formula	C₁₇H₁₉N₅O₄
CAS No.	4294-16-0	SDF	--
Smiles	OCC1OC(C(O)C1O)[N]2C=NC3=C(NCC4=CC=CC=C4)N=CN=C23
Storage (From the date of receipt)	3 years -20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C powder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 71 mg/mL ( (198.67 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 1 mg/mL

Water : Insoluble

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
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Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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