DNPH1i(N6-benzyladenosine)

Catalog No.E1093 Batch:E109301

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Technical Data

Formula

C17H19N5O4
Molecular Weight 357.36 CAS No. 4294-16-0
Solubility (25°C)* In vitro DMSO 71 mg/mL (198.67 mM)
Ethanol 1 mg/mL (2.79 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description N6-benzyladenosine (DNPH1i ) is an effective antiproliferative agent both in vitro and in vivo. It is a competitive inhibitor of DNPH1, limiting the activity of DNPH1 in vitro, and a competitive inhibitor of adenosine deaminase (ADA) in L-1210 cells.
In vitro

DNPH1i is the DNPH1 competitive inhibitor, that co-treatmented with hmdU selectively kills the HR-deficient cells, BRCA1-defective cells and PARPi-resistant BRCA1-mutant cell lines depleted of PARP1, sensitizing BRCA-deficient cells to PARPi or hmdU treatment.[2]

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    SUM149 BRCA1mut (parental) and WT (revertant) cell lines

  • Concentrations

    0.3 µM

  • Incubation Time

    8 days

  • Method

    SUM149 BRCA1mut (parental) and WT (revertant) cell lines are either untreated or treated with hmdU in the absence or presence of DNPH1i (0.3 μM) for 8 days. Viability is determined using CellTiter-Glo.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.