Home Metabolism Hydroxylase inhibitor - HIF modulator - Autophagy activator DMOG For research use only.

DMOG

Synonyms: Dimethyloxalylglycine

DMOG (Dimethyloxalylglycine) is an antagonist of α-ketoglutarate cofactor and inhibitor for HIF prolylhydroxylase, leading to stabilisation and accumulation of HIF-1α protein in the nucleus. DMOG enhances autophagy.Solutions are unstable and should be fresh-prepared.

DMOG Chemical Structure

DMOG Chemical Structure

CAS No. 89464-63-1

Purity & Quality Control

DMOG Related Products

Signaling Pathway

Hydroxylase Signaling Pathways

Hydroxylase Signaling Pathway

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human SR cell Growth inhibition assay Inhibition of human SR cell growth in a cell viability assay, IC50=4.57946 μM SANGER
human LB996-RCC cell Growth inhibition assay Inhibition of human LB996-RCC cell growth in a cell viability assay, IC50=5.6877 μM SANGER
human LOUCY cell Growth inhibition assay Inhibition of human LOUCY cell growth in a cell viability assay, IC50=10.3493 μM SANGER
human KS-1 cell Growth inhibition assay Inhibition of human KS-1 cell growth in a cell viability assay, IC50=11.2557 μM SANGER
human U-698-M cell Growth inhibition assay Inhibition of human U-698-M cell growth in a cell viability assay, IC50=13.3306 μM SANGER
human A3-KAW cell Growth inhibition assay Inhibition of human A3-KAW cell growth in a cell viability assay, IC50=15.8174 μM SANGER
human BL-70 cell Growth inhibition assay Inhibition of human BL-70 cell growth in a cell viability assay, IC50=15.9423 μM SANGER
human DMS-273 cell Growth inhibition assay Inhibition of human DMS-273 cell growth in a cell viability assay, IC50=15.965 μM SANGER
human CAL-39 cell Growth inhibition assay Inhibition of human CAL-39 cell growth in a cell viability assay, IC50=19.3277 μM SANGER
human KYSE-410 cell Growth inhibition assay Inhibition of human KYSE-410 cell growth in a cell viability assay, IC50=20.8197 μM SANGER
human YT cell Growth inhibition assay Inhibition of human YT cell growth in a cell viability assay, IC50=23.3984 μM SANGER
human LC-2-ad cell Growth inhibition assay Inhibition of human LC-2-ad cell growth in a cell viability assay, IC50=24.5573 μM SANGER
human COR-L105 cell Growth inhibition assay Inhibition of human COR-L105 cell growth in a cell viability assay, IC50=26.4436 μM SANGER
human CAL-12T cell Growth inhibition assay Inhibition of human CAL-12T cell growth in a cell viability assay, IC50=26.5502 μM SANGER
human KU812 cell Growth inhibition assay Inhibition of human KU812 cell growth in a cell viability assay, IC50=28.4579 μM SANGER
human OPM-2 cell Growth inhibition assay Inhibition of human OPM-2 cell growth in a cell viability assay, IC50=28.8227 μM SANGER
human SW982 cell Growth inhibition assay Inhibition of human SW982 cell growth in a cell viability assay, IC50=33.7007 μM SANGER
human HH cell Growth inhibition assay Inhibition of human HH cell growth in a cell viability assay, IC50=34.0235 μM SANGER
human RS4-11 cell Growth inhibition assay Inhibition of human RS4-11 cell growth in a cell viability assay, IC50=34.1806 μM SANGER
human BEN cell Growth inhibition assay Inhibition of human BEN cell growth in a cell viability assay, IC50=36.8187 μM SANGER
human CCRF-CEM cell Growth inhibition assay Inhibition of human CCRF-CEM cell growth in a cell viability assay, IC50=37.7729 μM SANGER
human EW-13 cell Growth inhibition assay Inhibition of human EW-13 cell growth in a cell viability assay, IC50=39.0481 μM SANGER
human NCI-H2228 cell Growth inhibition assay Inhibition of human NCI-H2228 cell growth in a cell viability assay, IC50=42.818 μM SANGER
human KE-37 cell Growth inhibition assay Inhibition of human KE-37 cell growth in a cell viability assay, IC50=43.2915 μM SANGER
human A4-Fuk cell Growth inhibition assay Inhibition of human A4-Fuk cell growth in a cell viability assay, IC50=45.0364 μM SANGER
human MZ1-PC cell Growth inhibition assay Inhibition of human MZ1-PC cell growth in a cell viability assay, IC50=45.9888 μM SANGER
human MLMA cell Growth inhibition assay Inhibition of human MLMA cell growth in a cell viability assay, IC50=46.3292 μM SANGER
human EB-3 cell Growth inhibition assay Inhibition of human EB-3 cell growth in a cell viability assay, IC50=49.5792 μM SANGER
human 293T cells  Function assay 2.5 mM Inhibition of JMJD2-mediated H3K36 demethylation in human 293T cells at 2.5 mM 19359167
Click to View More Cell Line Experimental Data

Biological Activity

Description DMOG (Dimethyloxalylglycine) is an antagonist of α-ketoglutarate cofactor and inhibitor for HIF prolylhydroxylase, leading to stabilisation and accumulation of HIF-1α protein in the nucleus. DMOG enhances autophagy.Solutions are unstable and should be fresh-prepared.
Targets
HIF prolyl hydroxylase [1]
In vitro
In vitro

DMOG shows only weakly active in the microsomal system, but efficiently suppresses hydroxyproline synthesis in intact cells. [1] DMOG reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC. [3]
Cell Research Cell lines VSMC cells
Concentrations ~100 μM
Incubation Time 72-96 hours
Method

To analyze DNA synthesis as an index of cellular proliferation, VSMC are plated in 48-well plates (5,000 per square centimeter) in growth medium, incubated overnight, and serum-deprived (1% FCS) for 24 h. Replicate wells are then stored at −70°C for baseline (day 0) cell counts, and fresh medium with or without growth factors is added to the remaining wells, which are incubated 72-96 h in 20 or 5% O2. Days 0 and 3 or 4 cell counts are determined by lysing cells in a buffer containing a fluorescent dye, which has minimal fluorescence by itself but fluoresces when bound to DNA or RNA. Absolute cell numbers are calculated by comparing the fluorescence of specimens with that of a standard curve similarly prepared using a known number of cells.
Experimental Result Images Methods Biomarkers Images PMID
Western blot HIF-1α p-CHK1 / CHK1 / p-p53 / p53 / p-CHK2 / CHK2 / p-ATM / ATM WNT11 / HIF-2α 29555474
Immunofluorescence HIF1α 28955984
In Vivo
In vivo

DMOG inhibits endogenous HIF inactivation, and induces angiogenesis in ischaemic skeletal muscles of mice. [2] Up-regulation of hypoxia-inducible factor-1α by DMOG enhances the cardioprotective effects of ischemic postconditioning in hyperlipidemic rats. [4]
Animal Research Animal Models Mice (C57Bl6)
Dosages 8 mg in 0.5 ml saline
Administration i.p.

Chemical Information & Solubility

Molecular Weight 175.14 Formula

C6H9NO5
CAS No. 89464-63-1 SDF Download DMOG SDF
Density 1.246 g/mL
Smiles COC(=O)CNC(=O)C(=O)OC
Storage (From the date of receipt) 3 years -20°C powder Solutions are unstable. Prepare fresh or purchase small, pre-packaged sizes. Repackage upon receipt.

In vitro
Batch:

DMSO : 35 mg/mL ( (199.84 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 35 mg/mL

Ethanol : 35 mg/mL


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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