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Dibutyryl-cAMP (Bucladesine)

Synonyms: dbcAMP

Catalog No.S7858 Purity:99.96%

Dibutyryl-cAMP (Bucladesine, dbcAMP) is a cell-permeable PKA activator by mimicing the action of endogenous cAMP. Dibutyryl-cAMP (Bucladesine) is also a phosphodiesterase (PDE) inhibitor.

Dibutyryl-cAMP (Bucladesine) Chemical Structure

CAS No. 16980-89-5

Purity & Quality Control

Batch: Purity: 99.96%

99.96

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Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
mouse RAW264.7 cells	Function assay		4 h	Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced TNFalpha production after 4 hrs by ELISA, IC50=28.9 μM	11000020
mouse S49 cells	Cytotoxicity assay	500 μM	20-49 h	Cytotoxicity against HGPRTase-deficient mouse S49 cells assessed as growth inhibition at 500 uM after 20 to 49 hrs by time-course study	221658
Click to View More Cell Line Experimental Data

Biological Activity

Description

Dibutyryl-cAMP (Bucladesine, dbcAMP) is a cell-permeable PKA activator by mimicing the action of endogenous cAMP. Dibutyryl-cAMP (Bucladesine) is also a phosphodiesterase (PDE) inhibitor.

Targets

PDE ^[5]

PKA ^[1]

In vitro
In vitro	Dibutyryl-cAMP inhibits neuronal glucose uptake via PKA activation. ^[1] In cultured rat hepatocytes, Dibutyryl-cAMP inhibits inducible nitric oxide synthase expression and NF-kappaB-binding activity. ^[2] Dibutyryl-cAMP also suppress TNFalpha-induced hepatocyte apoptosis by inhibiting FADD up-regulation. ^[3]
Cell Research	Cell lines	PC12 cells
	Concentrations	1 mM
	Incubation Time	24 h
	Method	Cells were incubated with 1 mM of db-cAMP.
Experimental Result Images	Methods	Biomarkers	Images	PMID
Experimental Result Images	Immunofluorescence	α-SMAD 2/3		19858184

In Vivo
In vivo	In a mouse model, bucladesine (600 nM/mouse, i.p.) reverses zinc chloride- and lead acetate-induced avoidance memory retention impairments. ^[4]
Animal Research	Animal Models	Mice
	Dosages	600 nM/mouse
	Administration	i.p.

References
[1] Prapong T, et al. Exp Neurol. 2001, 167(1), 59-64. [2] Harbrecht BG, et al. J Surg Res. 2001, 99(2), 258-264. [3] Wang Y, et al. Apoptosis. 2006, 11(3), 441-451. [4] Biol Trace Elem Res. 2015, DOI10.1007/s12011-015-0399-z [5] Chris Rundfeldt, et al. Arch Dermatol Res . 2012 May;304(4):313-7. [6] Shimojo M, et al. J Neurochem. 1998 Sep;71(3):1118-26. + Expand

References

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Chemical Information & Solubility

Molecular Weight	491.37	Formula	C₁₈H₂₃N₅NaO₈P
CAS No.	16980-89-5	SDF	Download Dibutyryl-cAMP (Bucladesine) SDF
Smiles	CCCC(=O)NC1=C2C(=NC=N1)N(C=N2)C3C(C4C(O3)COP(=O)(O4)[O-])OC(=O)CCC.[Na+]
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 98 mg/mL ( (199.44 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 98 mg/mL

Ethanol : 25 mg/mL

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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