Home GPCR & G Protein PKA activator 8-Bromo-cAMP For research use only.

8-Bromo-cAMP

Synonyms: 8-Br-Camp,8-Bromo-cAMP Sodium

8-bromo-cAMP (8-Br-Camp) is a cell permeable analog of cAMP that activates cyclic-AMP-dependent protein kinase with a Ka value of 0.05 μM; and a PKA activator.

8-Bromo-cAMP Chemical Structure

8-Bromo-cAMP Chemical Structure

CAS No. 76939-46-3

Purity & Quality Control

8-Bromo-cAMP Related Products

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
RAW 264.7 Function assay 50 μM 20 min the ADP-enhanced ERK signaling was significantly decreased. 27867196
HUVECs Function assay enhances capillary network formation 24493289
MC3T3-E1 Function assay 1 day significant increases in ALP activities 24493289
CHO  Function assay 0, 10, and 100 μmol/L 8-Br-cAMP increased [Ca2+]i in NCX1 transfectants at reverse mode 23564083
N2a cells Function assay 1-1,000 μM 24 h Aβ40, Aβ42 and sAPPα were significantly increased 23297063
Click to View More Cell Line Experimental Data

Biological Activity

Description 8-bromo-cAMP (8-Br-Camp) is a cell permeable analog of cAMP that activates cyclic-AMP-dependent protein kinase with a Ka value of 0.05 μM; and a PKA activator.
Targets
PKA [1]
0.05 μM(Ka)
In vitro
In vitro

8-Bromo-cAMP improves the reprogramming efficiency of human neonatal foreskin fibroblast (HFF1) cells. [1]

In bronchial epithelial cells, 8-Bromo-cAMP decreases HDE-stimulated tumor necrosis factor (TNF)-α-converting enzyme (TACE; ADAM-17) activity and subsequent TNF-α release. [2]
Cell Research Cell lines BEAS-2B cells
Concentrations 100 μM
Incubation Time 1 h
Method

Cells were treated with various concentrations of 8-Br-cAMP.

Chemical Information & Solubility

Molecular Weight 430.08 Formula

C10H10BrN5NaO6P
CAS No. 76939-46-3 SDF Download 8-Bromo-cAMP SDF
Smiles C1C2C(C(C(O2)N3C4=NC=NC(=C4N=C3Br)N)O)OP(=O)(O1)[O-].[Na+]
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 86 mg/mL ( (199.96 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 86 mg/mL

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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