Home GPCR & G Protein GPCR19 activator Deoxycholic acid For research use only.

Deoxycholic acid

Synonyms: Deoxycholate, Desoxycholic acid, Cholerebic, Cholorebic

Deoxycholic acid (Deoxycholate, Desoxycholic acid, Cholerebic, Cholorebic) is a cytolytic agent. The physiologic effect of deoxycholic acid is by means of decreased cell membrane integrity. Deoxycholic acid is specifically responsible for activating the G protein-coupled bile acid receptor TGR5 that stimulates brown adipose tissue (BAT) thermogenic activity.

Deoxycholic acid Chemical Structure

Deoxycholic acid Chemical Structure

CAS No. 83-44-3

Purity & Quality Control

Batch: S468901 DMSO]78 mg/mL]false]Ethanol]60 mg/mL]false]Water]Insoluble]false Purity: 99.94%
99.94

Deoxycholic acid Related Products

Biological Activity

Description Deoxycholic acid (Deoxycholate, Desoxycholic acid, Cholerebic, Cholorebic) is a cytolytic agent. The physiologic effect of deoxycholic acid is by means of decreased cell membrane integrity. Deoxycholic acid is specifically responsible for activating the G protein-coupled bile acid receptor TGR5 that stimulates brown adipose tissue (BAT) thermogenic activity.
In vitro
In vitro Deoxycholic acid inhibits miR-21 expression in primary rat hepatocytes in a dose-dependent manner, and increases miR-21 pro-apoptotic target programmed cell death 4 (PDCD4) and apoptosis. Deoxycholic acid decreases NF-κB activity, shown to represent an upstream mechanism leading to modulation of the miR-21/PDCD4 pathway[1].
Cell Research Cell lines Primary rat hepatocytes
Concentrations 25 to 200 μM
Incubation Time 24 h
Method

Primary rat hepatocytes were isolated from male rats (100 to 150 g) by collagenase perfusion. After isolation, hepatocytes were resuspended in complete Williams E medium and plated on BD Primaria™ culture dishes at 5 × 104 cells/cm2. Cells are kept at 37 °C in a humidified atmosphere of 5% CO2 for 4-6 h to allow attachment. Plates are then washed with phosphate buffered saline (PBS) 1× in order to remove dead cells and incubated in Williams E medium supplemented with 25 to 200 μM DCA or no addition (control) for 24 h. Primary rat hepatocytes are processed for total RNA and protein isolation, cell viability, cytotoxicity and caspase activity assays and Hoechst staining.
In Vivo
In vivo miR-21 expression is down-regulated upon short exposures to Deoxycholic acid, as is the downstream pathway, with concomitant PIDD processing and activation of caspase-2, which contributes to DCA-induced liver damage[1].
Animal Research Animal Models Male Wistar rats
Dosages 125 mg/kg
Administration p.o.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02654496 Completed
Obesity
North Dakota State University
 January 2016 --
NCT01865812 Completed
Primary Biliary Cirrhosis
Intercept Pharmaceuticals
 December 3 2013 Phase 2
NCT01319916 Completed
Healthy
Kythera Biopharmaceuticals
 December 2010 Phase 1

Chemical Information & Solubility

Molecular Weight 392.57 Formula

C24H40O4
CAS No. 83-44-3 SDF Download Deoxycholic acid SDF
Smiles CC(CCC(=O)O)C1CCC2C1(C(CC3C2CCC4C3(CCC(C4)O)C)O)C
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 78 mg/mL ( (198.69 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 60 mg/mL

Water : Insoluble


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In vivo
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