Home Transmembrane Transporters ATPase inhibitor - Fungal inhibitor Ciclopirox ethanolamine For research use only.

Ciclopirox ethanolamine

Synonyms: Ciclopirox olamine, HOE 296,Ciclopiroxolamine

Ciclopirox ethanolamine (Ciclopirox olamine, HOE 296,Ciclopiroxolamine) is a broad-spectrum antifungal agent working as an iron chelator.

Ciclopirox ethanolamine Chemical Structure

Ciclopirox ethanolamine Chemical Structure

CAS No. 41621-49-2

Purity & Quality Control

Batch: S301901 DMSO]40 mg/mL]false]Ethanol]30 mg/mL]false]Water]Insoluble]false Purity: 99.91%
99.91

Ciclopirox ethanolamine Related Products

Signaling Pathway

ATPase Signaling Pathways

ATPase Signaling Pathway

Biological Activity

Description Ciclopirox ethanolamine (Ciclopirox olamine, HOE 296,Ciclopiroxolamine) is a broad-spectrum antifungal agent working as an iron chelator.
Features Exhibits antifungal activity via a specific iron limiation mechanism, with no single case of fungal resistance reported.
Targets
ATPase [3]
In vitro
In vitro

Ciclopirox significantly inhibits the growth of C. albicans strain SC5314 cells, with MIC80s of 1.0-2.0 μg/mL, growth decreased dramatically at the concentrations of >0.6 μg/mL, and almost complete growth inhibition at concentration of 0.7 μg/mL, unlike fluconazole which shows a much wider range of concentrations with intermediate inhibition. Like iron chelator bipyridine, Ciclopirox reduces cell growth by binding to iron ions, which can be reversed by addition of FeCl3. Moreover, Ciclopirox treatment at subinhibitory concentration (0.6 μg/mL) only moderately reduces the virulence genes such as genes encoding secreted proteinases or lipases, but leads to a distinct up- or down-regulation of genes encoding iron permeases or transporters (FTR1, FTR2, and FTH1), a copper permease (CCC2), an iron reductase (CFL1), and a siderophore transporter (SIT1). Although the Candida drug resistance genes CDR1 and CDR2 are up-regulated after Ciclopirox treatment, no change in resistance or increased tolerance could be observed even after an incubation period of 6 months, in contrast to fluconazole in which the MICs for cells noticeably increase after 2 months. [1] Ciclopirox inhibits the growth of Aspergillus fumigatus strain B5233 with IC50 of 4.22 μM, more potently compared with deferiprone with IC50 of 1.29 mM. [2]
Cell Research Cell lines C. albicans strain SC5314
Concentrations Dissolved in DMSO, final concentrations 10 μg/mL
Incubation Time 1-10 hours
Method

Sabouraud glucose medium (2%) is used for cell culture growth, and RPMI 2% glucose medium and 2% Sabouraud glucose medium are used for MIC determinations. For cell culture growth curves, 220 mL of 2% Sabouraud glucose medium containing different concentrations of Ciclopirox are inoculated with 105 cells/mL, and the mixture is shaken at 160 rpm and 37 °C for 1-10 hours. Growth is measured photometrically at 630 nm. FeCl3 or 2,2'-bipyridine is added to the medium at different concentrations for inhibition studie
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00990587 Completed
Hematologic Malignancy|Acute Lymphocytic Leukemia|Chronic Lymphocytic Leukemia|Myelodysplasia|Acute Myeloid Leukemia|Chronic Myelogenous Leukemia|Hodgkin''s Disease
University Health Network Toronto|The Leukemia and Lymphoma Society
 October 2009 Phase 1
NCT01646580 Terminated
Dermatomycoses
Ferrer Internacional S.A.
 October 2008 Phase 4

Chemical Information & Solubility

Molecular Weight 268.35 Formula

C12H17NO2.C2H7NO
CAS No. 41621-49-2 SDF Download Ciclopirox ethanolamine SDF
Smiles CC1=CC(=O)N(C(=C1)C2CCCCC2)O.C(CO)N
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 40 mg/mL ( (149.05 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 30 mg/mL

Water : Insoluble


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