Home Cell Cycle Rho inhibitor CCG-1423 For research use only.

CCG-1423

CCG-1423 is a specific RhoA pathway inhibitor, which inhibits SRF-mediated transcription.

CCG-1423 Chemical Structure

CCG-1423 Chemical Structure

CAS No. 285986-88-1

Purity & Quality Control

Batch: S771901 DMSO]90 mg/mL]false]Ethanol]4 mg/mL]false]Water]Insoluble]false Purity: 99.98%
99.98

CCG-1423 Related Products

Signaling Pathway

Rho Signaling Pathways

Rho Signaling Pathway

Biological Activity

Description CCG-1423 is a specific RhoA pathway inhibitor, which inhibits SRF-mediated transcription.
Targets
RhoA [1]
In vitro
In vitro CCG-1423 selectively inhibits SRF-mediated transcription activated by Rho pathway signaling, and specifically inhibits LPA-stimulated DNA synthesis. CCG-1423 also selectively inhibits the proliferation of RhoC-overexpressing melanoma cells (A375M2 and SK-Mel-147), and strongly suppresses the Rho-dependent invasion by PC-3 cells. [1] CCG-1423 in combination with LY294002 enhances differentiation of mouse embryonic stem cells into intermediate mesoderm through BMP7-positive cells. [2] In H9c2 cells, CCG-1423 inhibits MRTF nuclear localization, and completely blocks STARS proximal reporter activity. [3] CCG-1423, as a Rho/MRTF/SRF pathway inhibitor, also represses both matrix-stiffness and TGF-beta-induced fibrogenesis in human colonic myofibroblasts. [4]
Cell Research Cell lines Melanoma lines that overexpress RhoC (A375M2 and SK-Mel-147), low RhoC-expressing lines (A375 and SK-Mel-28), transformed (SW962, PC-3, and SKOV-3) and nontransformed (WI-38) cell lines.
Concentrations 300 nM
Incubation Time 72 hours
Method Cells in normal culture medium are plated (2,000 per well) in a 96-well plate coated with laminin. After attachment, the medium is replaced with serum-free medium (0% FBS) with 30 μmol/L LPA with or without 300 nM CCG-1423. Fresh LPA with or without CCG-1423 is added at day 5 to ensure that LPA and compound are present throughout the experiment. On day 8, WST-1 reagent is added to the wells for 1 h and absorbance at 450 nm is read using a Victor plate reader.

Chemical Information & Solubility

Molecular Weight 454.75 Formula

C18H13ClF6N2O3
CAS No. 285986-88-1 SDF Download CCG-1423 SDF
Smiles CC(C(=O)NC1=CC=C(C=C1)Cl)ONC(=O)C2=CC(=CC(=C2)C(F)(F)F)C(F)(F)F
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 90 mg/mL ( (197.91 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 4 mg/mL

Water : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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