Home Cell Cycle Rho inhibitor NSC 23766 trihydrochloride For research use only.

NSC 23766 trihydrochloride

NSC 23766 trihydrochloride is an inhibitor of Rac GTPase targeting Rac activation by guanine nucleotide exchange factors (GEFs) with IC50 of ~50 μM in a cell-free assay; does not inhibit the closely related targets, Cdc42 or RhoA.

NSC 23766 trihydrochloride Chemical Structure

NSC 23766 trihydrochloride Chemical Structure

CAS No. 1177865-17-6

Purity & Quality Control

NSC 23766 trihydrochloride Related Products

Signaling Pathway

Rho Signaling Pathways

Rho Signaling Pathway

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
RA4 Function Assay 50 μM 24 h inhibits Matrigel invasion 17622308
RA3 Function Assay 50 μM 24 h inhibits Matrigel invasion 17622308
RA2 Function Assay 50 μM 24 h inhibits Matrigel invasion 17622308
RA1 Function Assay 50 μM 24 h inhibits Matrigel invasion 17622308
RA-FLS (RA2)  Growth Inhibition Assay 25/50 μM 1-9 d inhibits cell growth in both dose and time dependent manner 17622308
IEC-6  Function Assay 120 µM 4/6/8 h prevents the increased activation of FAK at 6 and 8 h 20448461
MDA-MB-231  Function Assay 50/100 μM 48 h induces a dose-dependent decrease in phosphorylation of p65 subunit 20515940
MDA-MB-468 Function Assay 50/100 μM 48 h induces a dose-dependent decrease in phosphorylation of p65 subunit 20515940
MDA-MB-231  Function Assay 50/100 μM 24 h increases phosphorylation of JNK in a dose dependent manner 20515940
MDA-MB-468 Function Assay 50/100 μM 24 h increases phosphorylation of JNK in a dose dependent manner 20515940
MDA-MB-468 Function Assay 100 μM 24 h inhibits caspase-3 activation  20515940
MDA-MB-468 Apoptosis Assay 50/100 μM 24 h induces apoptosis 20515940
T47D Function Assay 100 μM 48 h increases the cell number in G1 phase and decreases the cell number in S and G2-M phases  20515940
MCF7 Function Assay 100 μM 48 h increases the cell number in G1 phase and decreases the cell number in S and G2-M phases  20515940
MDA-MB-231  Function Assay 100 μM 48 h increases the cell number in G1 phase and decreases the cell number in S and G2-M phases  20515940
MDA-MB-231 Function Assay 0-100 μM 24 h selectively inhibits Rac1 activation without interfering with the activity of the closely related small GTPase Cdc42 20515940
MDA-MB-231 Cytotoxicity Assay 0-100 μM 48 h decreases cell viability in a dose dependent manner 20515940
MDA-MB-468 Cytotoxicity Assay 0-100 μM 48 h decreases cell viability in a dose dependent manner 20515940
T47D Cytotoxicity Assay 0-100 μM 48 h decreases cell viability in a dose dependent manner 20515940
MCF7 Cytotoxicity Assay 0-100 μM 48 h decreases cell viability in a dose dependent manner 20515940
SKBR3-pMKO.1 Function Assay 50 μM 24 h inhibits Rac1 activation 21943825
SKBR3 Function Assay 50 μM 24 h inhibits Rac1 activation 21943825
NCI-H1703 Function Assay 0-500 μM 24 h diminishes basal NF-κB activity dose dependently  22549160
NCI-H1703 Function Assay 100 μg/ml 24 h slows progression through the G1 phase of the cell cycle 22549160
NCI-H1703 Growth Inhibition Assay 0-500 μM 24 h inhibits cell growth in a dose dependent manner 22549160
T98MG Function Assay 50 mM 24 h DMSO enhances the antimigratory effect of erlotinib 23832120
A172MG Function Assay 50 mM 24 h DMSO enhances the antimigratory effect of erlotinib 23832120
U87MG Function Assay 50 mM 24 h DMSO enhances the antimigratory effect of erlotinib 23832120
PC40 Cell Viability Assay 50 mM 144 h DMSO exhibits synergistic antiproliferative effects combined treatment with erlotinib  23832120
PC38 Cell Viability Assay 50 mM 144 h DMSO exhibits synergistic antiproliferative effects combined treatment with erlotinib  23832120
T98MG Cell Viability Assay 50 mM 144 h DMSO exhibits synergistic antiproliferative effects combined treatment with erlotinib  23832120
A172MG Cell Viability Assay 50 mM 144 h DMSO exhibits synergistic antiproliferative effects combined treatment with erlotinib  23832120
U87MG Cell Viability Assay 50 mM 144 h DMSO exhibits synergistic antiproliferative effects combined treatment with erlotinib  23832120
Ki-67+ CLL Growth Inhibition Assay 50 µM 5 d decreases the number of Ki-67+ CLL cells 24501217
NIH3T3  Growth Inhibition Assay 100 μM 24 h has no significant impact on cell viability 25037060
U2-OS Function Assay 100 μM 24 h DMSO induces cell cycle arrest in the G1 phase  25109327
SW480  Function Assay 100 μM 24 h DMSO induces cell cycle arrest in the G1 phase  25109327
A431 Function Assay 100 μM 24 h DMSO induces cell cycle arrest in the G1 phase  25109327
U2-OS Growth Inhibition Assay 100 μM 24/48/72 h inhibits cell growth in a time dependent manner 25109327
SW480  Growth Inhibition Assay 100 μM 24/48/72 h inhibits cell growth in a time dependent manner 25109327
A431 Growth Inhibition Assay 100 μM 24/48/72 h inhibits cell growth in a time dependent manner 25109327
RBMECs Function Assay 100 μM  30 min blockes 6Bnz-cAMP-mediated activation of Rac1 in EMAP-II-treated RBMECs 26358039
human aortic smooth muscle cells Function Assay 50 uM Inhibition of Rac1 binding to Pak1 in human aortic smooth muscle cells at 50 uM by SDS-PAGE based chemiluminescence 19527032
human aortic smooth muscle cells Function Assay 100 μM Inhibition of Rac1 binding to Pak1 in human aortic smooth muscle cells at 100 uM by SDS-PAGE based chemiluminescence 19527032
Click to View More Cell Line Experimental Data

Biological Activity

Description NSC 23766 trihydrochloride is an inhibitor of Rac GTPase targeting Rac activation by guanine nucleotide exchange factors (GEFs) with IC50 of ~50 μM in a cell-free assay; does not inhibit the closely related targets, Cdc42 or RhoA.
Targets
Rac GTPase [1]
(Cell-free assay)
50 μM

Chemical Information & Solubility

Molecular Weight 530.97 Formula

C24H35N7.3ClH
CAS No. 1177865-17-6 SDF Download NSC 23766 trihydrochloride SDF
Smiles CCN(CC)CCCC(C)NC1=NC(=CC(=N1)NC2=CC3=C(C=C(N=C3C=C2)C)N)C.Cl.Cl.Cl
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 106 mg/mL ( (199.63 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 106 mg/mL

Ethanol : 5 mg/mL


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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