Home Apoptosis IAP inhibitor BV-6 For research use only.

BV-6

BV-6 is a SMAC mimetic, dual cIAP and XIAP inhibitor.

BV-6 Chemical Structure

BV-6 Chemical Structure

CAS No. 1001600-56-1(freebase)

Purity & Quality Control

BV-6 Related Products

Signaling Pathway

IAP Signaling Pathways

IAP Signaling Pathway

Biological Activity

Description BV-6 is a SMAC mimetic, dual cIAP and XIAP inhibitor.
Targets
cIAP [1] XIAP [1]
In vitro
In vitro

BV6 inhibits the cell viability of HCC193 NSCLC cells with IC50 of 7.2 μM, induces apoptosis in both HCC193 and H460 cell lines, and also significantly enhances the radiosensitivity of these cell lines through activation of cleaved caspase-8 and cleaved caspase-9, respectively. [1]

In immature dendritic cells, BV-6 treatment results in moderate activation of the classical NF-kB pathway. [2]

Furthermore, BV-6 increases CIK cell-mediated lysis of hematological (H9, THP-1, and Tanoue) and solid malignancies (RH1, RH30, and TE671). BV-6 also enhances apoptosis of peripheral blood mononuclear cells and most notably has an inhibitory effect on immune cells limiting their cytotoxic potential. [3]
Cell Research Cell lines HCC193 and H460 cells
Concentrations ~30 μM
Incubation Time ~48 hours
Method

Cell viability is measured using the CellTiter 96® Aqueous Non-Radioactive Cell Proliferation Assay kit. 5000 cells/well are seeded into 96-well plates in triplicate. Following adhesion of cells to the wells, increasing concentrations of BV6 are added into different wells. Control groups are exposed to the same concentration of DMSO. The final concentrations of 333 μg/mL MTS and 25 μM PMS are added to each well 24 hours later. After two hours incubation at 37 °C in humidified 5% CO2, plates are read at the absorbance of 490-nm on a microplate reader. Relative cell viability of an individual sample is calculated by normalizing their absorbance to that of the corresponding control. IC50 values are calculated using Prism 5.01. For the TNFα neutralizing antibody assay, cells are exposed to 1 and 5 μM BV6 and the assay is performed 24 hours later. Plates are read at the absorbance of 490-nm on a microplate reader
Experimental Result Images Methods Biomarkers Images PMID
Western blot cIAP1 / XIAP RIP1 / FADD / Caspase-8 p52 / RelB / p50 / p-p65 / Lamin B1 21760551
Growth inhibition assay Cell death 26775704
In Vivo
In vivo

BV6 is an IAP antagonist.
Animal Research Animal Models Female BALB/c mice
Dosages 10 mg/kg
Administration i.p.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05425342 Completed
Electrocardiogram
University Hospital Basel Switzerland|University of Basel
 December 12 2020 Not Applicable

Chemical Information & Solubility

Molecular Weight 1205.57 Formula

C70H96N10O8.xCF3COOH
CAS No. 1001600-56-1(freebase) SDF Download BV-6 SDF
Smiles CC(C(=O)NC(C1CCCCC1)C(=O)N2CCCC2C(=O)NC(C(C3=CC=CC=C3)C4=CC=CC=C4)C(=O)NCCCCCCNC(=O)C(C(C5=CC=CC=C5)C6=CC=CC=C6)NC(=O)C7CCCN7C(=O)C(C8CCCCC8)NC(=O)C(C)NC)NC
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (82.94 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 100 mg/mL

Ethanol : 100 mg/mL


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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