BV-6

Catalog No.S7597 Batch:S759704

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Technical Data

Formula

C70H96N10O8.xCF3COOH
Molecular Weight 1205.57 CAS No. 1001600-56-1(freebase)
Solubility (25°C)* In vitro DMSO 100 mg/mL (82.94 mM)
Water 100 mg/mL (82.94 mM)
Ethanol 100 mg/mL (82.94 mM)
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
5.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to make it clear; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description BV-6 is a SMAC mimetic, dual cIAP and XIAP inhibitor.
Targets
cIAP [1] XIAP [1]
In vitro

BV6 inhibits the cell viability of HCC193 NSCLC cells with IC50 of 7.2 μM, induces apoptosis in both HCC193 and H460 cell lines, and also significantly enhances the radiosensitivity of these cell lines through activation of cleaved caspase-8 and cleaved caspase-9, respectively. [1]

In immature dendritic cells, BV-6 treatment results in moderate activation of the classical NF-kB pathway. [2]

Furthermore, BV-6 increases CIK cell-mediated lysis of hematological (H9, THP-1, and Tanoue) and solid malignancies (RH1, RH30, and TE671). BV-6 also enhances apoptosis of peripheral blood mononuclear cells and most notably has an inhibitory effect on immune cells limiting their cytotoxic potential. [3]
In vivo

BV6 is an IAP antagonist.

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    HCC193 and H460 cells

  • Concentrations

    ~30 μM

  • Incubation Time

    ~48 hours

  • Method

    Cell viability is measured using the CellTiter 96® Aqueous Non-Radioactive Cell Proliferation Assay kit. 5000 cells/well are seeded into 96-well plates in triplicate. Following adhesion of cells to the wells, increasing concentrations of BV6 are added into different wells. Control groups are exposed to the same concentration of DMSO. The final concentrations of 333 μg/mL MTS and 25 μM PMS are added to each well 24 hours later. After two hours incubation at 37 °C in humidified 5% CO2, plates are read at the absorbance of 490-nm on a microplate reader. Relative cell viability of an individual sample is calculated by normalizing their absorbance to that of the corresponding control. IC50 values are calculated using Prism 5.01. For the TNFα neutralizing antibody assay, cells are exposed to 1 and 5 μM BV6 and the assay is performed 24 hours later. Plates are read at the absorbance of 490-nm on a microplate reader
Animal Study:

[4]
  • Animal Models

    Female BALB/c mice

  • Dosages

    10 mg/kg

  • Administration

    i.p.

Customer Product Validation

, , FASEB J, 2017, 31(11):4707-4719

Data from [Data independently produced by , , Clin Cancer Res, 2018, doi:10.1158/1078-0432.CCR-18-1548]

Selleck's BV-6 has been cited by 71 publications

Lymph node and tumor-associated PD-L1+ macrophages antagonize dendritic cell vaccines by suppressing CD8+ T cells [ Cell Rep Med, 2024, 5(1):101377] PubMed: 38232703
RIPK1 protects naive and regulatory T cells from TNFR1-induced apoptosis [ Cell Death Differ, 2024, 10.1038/s41418-024-01301-w] PubMed: 38734851
Starvation-induced phosphorylation activates gasdermin A to initiate pyroptosis [ Cell Rep, 2024, 43(9):114728] PubMed: 39264808
IE1 of Human Cytomegalovirus Inhibits Necroptotic Cell Death via Direct and Indirect Modulation of the Necrosome Complex [ Viruses, 2024, 16(2)290] PubMed: 38400065
Necroptosis-based glioblastoma prognostic subtypes: implications for TME remodeling and therapy response [ Ann Med, 2024, 56(1):2405079] PubMed: 39387496
Phase separation of FSP1 promotes ferroptosis [ Nature, 2023, 619(7969):371-377] PubMed: 37380771
Telomere-to-mitochondria signalling by ZBP1 mediates replicative crisis [ Nature, 2023, 614(7949):767-773] PubMed: 36755096
Phase separation of FSP1 promotes ferroptosis [ Nature, 2023, 619(7969):371-377] PubMed: 37380771
Telomere-to-mitochondria signalling by ZBP1 mediates replicative crisis [ Nature, 2023, 614(7949):767-773] PubMed: 36755096
Vemurafenib inhibits necroptosis in normal and pathological conditions as a RIPK1 antagonist [ Cell Death Dis, 2023, 14(8):555] PubMed: 37620300

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.