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Blasticidin S Hydrochloride Nucleoside antibiotic

Name: Blasticidin S Hydrochloride
Brand: Selleck Chemicals
SKU: S7419
Availability: InStock
Rating: 5 (32 reviews)

Cat.No.S7419

Blasticidin S HCl is a nucleoside antibiotic isolated from Stretomyces girseochromogenes. It acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation. It is used to select transfected cells carrying bsr or BSD resistance genes.

Chemical Structure

Molecular Weight: 458.9

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability

Quality Control

Batch: Purity: 99.78%

99.78

Related Targets	DNA/RNA Synthesis HDAC Topoisomerase PPAR PARP Sirtuin ATM/ATR Casein Kinase eIF MDM2/MDMX DNA-PK DNA alkylator DHFR Telomerase Nucleoside Analog/Antimetabolite Thymidylate Synthase CRISPR/Cas9 RAD51 BMI-1 LIM kinase RNR NUDIX High-mobility Group OGG1 Alkylating Agent MTH1 G-quadruplex SMN FENs WRN
Related Products	Ribostamycin Sulfate

Chemical Information, Storage & Stability

Molecular Weight	458.9	Formula	C₁₇H₂₇ClN₈O₅	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	3513-03-9	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	CN(CCC(CC(=O)NC1C=CC(OC1C(=O)O)N2C=CC(=NC2=O)N)N)C(=N)N.Cl

Solubility

In vitro
Batch:

Water : 46 mg/mL

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Clear solution	5%H2O 1 40%PEG 300 2 5%Tween 80 3 50%H2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	4.600mg/ml (10.02mM)	Taking the 1 mL working solution as an example, add 50 μL 92 mg/mL clarified H2O stock solution to 400 μL PEG300, mix evenly to clarify; add 50 μL Tween-80 to the above system, mix evenly to clarify; Then continue to add 500 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	protein synthesis ^[1] DNA synthesis ^[1]
In vitro	Blasticidin S inhibits protein synthesis by acting on the step of peptide transfer from peptidyl-sRNA to incoming aminoacyl-sRNA. ^[1] Blasticidin S also significantly inhibits DNA synthesis independent of their effect on protein synthesis. ^[2] Blasticidin S kills the cells more rapidly than G418, which makes blasticidin S-resistance gene (bsr) a selectable marker for mammalian cells. ^[3] Besides, Blasticidin S also efficiently binds Cu(II) ions and prevents DNA from metal-induced damage. ^[4]
In vivo	Blasticidin S HCl is an antibiotic isolated from Stretomyces girseochromogenes that acts as a protein synthesis inhibitor. It is used to select transfected cells carrying bsr or BSD resistance genes.
References	https://pubmed.ncbi.nlm.nih.gov/4860846/ https://pubmed.ncbi.nlm.nih.gov/849443/ https://pubmed.ncbi.nlm.nih.gov/1720391/ https://pubmed.ncbi.nlm.nih.gov/23880034/ https://pubmed.ncbi.nlm.nih.gov/31591565/ https://pubmed.ncbi.nlm.nih.gov/3346189/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287960/

Applications

Methods	Biomarkers	PMID
Western blot	p-IκBα / T-IκBα / β-actin	16184196
Growth inhibition assay	Cell viability	24782309
mRNA blot	MGG_00447 / MGG_03736 / MGG_04855 / MGG_09931 / Beta tubulin MGG_00447 / MGG_03736 / MGG_04855 / MGG_09931 / 18s rRNA	26889110

Tech Support

Handling Instructions

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