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Blasticidin S HCl

Blasticidin S HCl is a nucleoside antibiotic isolated from Stretomyces girseochromogenes. It acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation. It is used to select transfected cells carrying bsr or BSD resistance genes.

Blasticidin S HCl Chemical Structure

Blasticidin S HCl Chemical Structure

CAS No. 3513-03-9

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Biological Activity

Description Blasticidin S HCl is a nucleoside antibiotic isolated from Stretomyces girseochromogenes. It acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation. It is used to select transfected cells carrying bsr or BSD resistance genes.
Targets
protein synthesis [1] DNA synthesis [1]
In vitro
In vitro

Blasticidin S inhibits protein synthesis by acting on the step of peptide transfer from peptidyl-sRNA to incoming aminoacyl-sRNA. [1]

Blasticidin S also significantly inhibits DNA synthesis independent of their effect on protein synthesis. [2]

Blasticidin S kills the cells more rapidly than G418, which makes blasticidin S-resistance gene (bsr) a selectable marker for mammalian cells. [3]

Besides, Blasticidin S also efficiently binds Cu(II) ions and prevents DNA from metal-induced damage. [4]
Cell Research Cell lines HEK293T cells
Concentrations 5 µg/ml
Incubation Time 7 days
Method

Lentivirus infected cells were maintained in 5 μg/ml blasticidin containing culture medium for 7d, with fresh medium exchange every day.
Experimental Result Images Methods Biomarkers Images PMID
Western blot p-IκBα / T-IκBα / β-actin 16184196
Growth inhibition assay Cell viability 24782309
mRNA blot MGG_00447 / MGG_03736 / MGG_04855 / MGG_09931 / Beta tubulin MGG_00447 / MGG_03736 / MGG_04855 / MGG_09931 / 18s rRNA 26889110
In Vivo
In vivo

Blasticidin S HCl is an antibiotic isolated from Stretomyces girseochromogenes that acts as a protein synthesis inhibitor. It is used to select transfected cells carrying bsr or BSD resistance genes.

Chemical Information & Solubility

Molecular Weight 458.9 Formula

C17H27ClN8O5
CAS No. 3513-03-9 SDF Download Blasticidin S HCl SDF
Smiles CN(CCC(CC(=O)NC1C=CC(OC1C(=O)O)N2C=CC(=NC2=O)N)N)C(=N)N.Cl
Storage (From the date of receipt)

In vitro
Batch:

Water : 92 mg/mL

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : Insoluble


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In vivo
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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