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Technical Data
| Formula | C17H27ClN8O5 |
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| Molecular Weight | 458.9 | CAS No. | 3513-03-9 | |
| Solubility (25°C)* | In vitro | Water | 91 mg/mL (198.3 mM) | |
| DMSO | Insoluble | |||
| Ethanol | Insoluble | |||
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.) |
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Biological Activity
| Description | Blasticidin S HCl is a nucleoside antibiotic isolated from Stretomyces girseochromogenes. It acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation. It is used to select transfected cells carrying bsr or BSD resistance genes. | ||
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| In vitro | Blasticidin S inhibits protein synthesis by acting on the step of peptide transfer from peptidyl-sRNA to incoming aminoacyl-sRNA. [1] Blasticidin S also significantly inhibits DNA synthesis independent of their effect on protein synthesis. [2] Blasticidin S kills the cells more rapidly than G418, which makes blasticidin S-resistance gene (bsr) a selectable marker for mammalian cells. [3] Besides, Blasticidin S also efficiently binds Cu(II) ions and prevents DNA from metal-induced damage. [4] |
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| In vivo | Blasticidin S HCl is an antibiotic isolated from Stretomyces girseochromogenes that acts as a protein synthesis inhibitor. It is used to select transfected cells carrying bsr or BSD resistance genes. |
Protocol (from reference)
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References
Selleck's Blasticidin S HCl has been cited by 29 publications
| STK19 is a transcription-coupled repair factor that participates in UVSSA ubiquitination and TFIIH loading [ Nucleic Acids Res, 2024, gkae787] | PubMed: 39353615 |
| Targeting the HSP47-collagen axis inhibits brain metastasis by reversing M2 microglial polarization and restoring anti-tumor immunity [ Cell Rep Med, 2024, 5(5):101533] | PubMed: 38744278 |
| BMP suppresses Wnt signaling via the Bcl11b-regulated NuRD complex to maintain intestinal stem cells [ EMBO J, 2024, ] | PubMed: 39433900 |
| YEATS domain-containing protein GAS41 regulates nuclear shape by working in concert with BRD2 and the mediator complex in colorectal cancer [ Pharmacol Res, 2024, 206:107283] | PubMed: 38964523 |
| Chicoric Acid Ameliorated Beta-Amyloid Pathology and Enhanced Expression of Synaptic-Function-Related Markers via L1CAM in Alzheimer's Disease Models [ Int J Mol Sci, 2024, 25(6)3408] | PubMed: 38542381 |
| Repression of LSD1 potentiates homologous recombination-proficient ovarian cancer to PARP inhibitors through down-regulation of BRCA1/2 and RAD51 [ Nat Commun, 2023, 10.1038/s41467-023-42850-x] | PubMed: 37973845 |
| Genetically encoded photocatalytic protein labeling enables spatially-resolved profiling of intracellular proteome [ Nat Commun, 2023, 14(1):2978] | PubMed: 37221179 |
| USP36 stabilizes nucleolar Snail1 to promote ribosome biogenesis and cancer cell survival upon ribotoxic stress [ Nat Commun, 2023, 14(1):6473] | PubMed: 37833415 |
| USP36 stabilizes nucleolar Snail1 to promote ribosome biogenesis and cancer cell survival upon ribotoxic stress [ Nat Commun, 2023, 14(1):6473] | PubMed: 37833415 |
| Histone H3 lysine 27 crotonylation mediates gene transcriptional repression in chromatin [ Mol Cell, 2023, 83(13):2206-2221.e11] | PubMed: 37311463 |
RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.
NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.