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ACSS2 inhibitor

Name: ACSS2 inhibitor
Brand: Selleck Chemicals
SKU: S8588
Rating: 5 (19 reviews)

Synonyms: Ac-CoA Synthase Inhibitor1

Catalog No.S8588 Purity:99.69%

ACSS2 inhibitor is the most potent and specific inhibitor of acetate-dependent acetyl-CoA synthetase 2 (ACSS2). ACSS2 inhibitor also inhibits the respiratory syncytial virus (RSV).

ACSS2 inhibitor Chemical Structure

CAS No. 508186-14-9

Purity & Quality Control

Batch: Purity: 99.69%

99.69

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Biological Activity

Description

ACSS2 inhibitor is the most potent and specific inhibitor of acetate-dependent acetyl-CoA synthetase 2 (ACSS2). ACSS2 inhibitor also inhibits the respiratory syncytial virus (RSV).

Targets

ACSS2 ^[1]
(Cell-free assay)

~0.6 μM

In vitro
In vitro	This quinoxaline compound inhibits the ability of HepG2 cells to incorporate [14C]acetate into lipids with IC50 = 6.8 μM. The quinoxaline is also able to inhibit HepG2 utilization of [14C]acetate for histone acetylation with IC50 = 5.5 μM^[1].
Cell Research	Cell lines	MDA-MB-231-R cells
	Concentrations	10 uM
	Incubation Time	6 h
	Method	10 µM of ACSS2 inhibitor was added in flag-WT or KR-STAT3 expressing MDA-MB-231-R cells and incubated for 6 h.

References

Chemical Information & Solubility

Molecular Weight	410.51	Formula	C₂₀H₁₈N₄O₂S₂
CAS No.	508186-14-9	SDF	Download ACSS2 inhibitor SDF
Smiles	COCCNC(=O)NC1=CC2=C(C=C1)N=C(C(=N2)C3=CC=CS3)C4=CC=CS4
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 15 mg/mL ( (36.53 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 2 mg/mL

Water : Insoluble

Molecular Weight Calculator

In vivo Batch: Add solvents to the product individually and in order.					In vivo Formulation Calculator
	Clear solution	10%DMSO 1 90%Corn oil	0.2mg/ml (0.49mM)	Taking the 1 mL working solution as an example, add 100 μL of 2 mg/ml clear DMSO stock solution to 900 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.	In vivo Formulation Calculator
Clear solution	5%DMSO 1 Corn oil	1.03mg/ml (2.51mM)	Taking the 1 mL working solution as an example, add 50 μL of 20.5 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 40% PEG 300 2 55%ddH2O	1.25mg/ml (3.04mM)	Taking the 1 mL working solution as an example, add 50 μL of 25 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 550 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

Preparing Stock Solutions

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

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Frequently Asked Questions

Question 1:
Regarding the ACSS2 inhibitor S8588, how do I prevent the formation of crystals in cell culture?

Answer:
For cell culture experiments, if there is precipitate or crystal formations after diluting to 1:1000 please reformulate. You can try the following ratio for formulation with 0.1% stock solution (in DMSO) + 1% PEG300 + 99% medium.

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