ACSS2 inhibitor

Catalog No.S8588 Batch:S858803

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Technical Data

Formula

C20H18N4O2S2
Molecular Weight 410.51 CAS No. 508186-14-9
Solubility (25°C)* In vitro DMSO 82 mg/mL (199.75 mM)
Ethanol 5 mg/mL (12.17 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO Corn oil
1.03mg/ml Taking the 1 mL working solution as an example, add 50 μL of 20.5 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 40% PEG 300 55%ddH2O
1.25mg/ml Taking the 1 mL working solution as an example, add 50 μL of 25 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 550 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description ACSS2 inhibitor is the most potent and specific inhibitor of acetate-dependent acetyl-CoA synthetase 2 (ACSS2). ACSS2 inhibitor also inhibits the respiratory syncytial virus (RSV).
Targets
ACSS2 [1]
(Cell-free assay)
~0.6 μM
In vitro

This quinoxaline compound inhibits the ability of HepG2 cells to incorporate [14C]acetate into lipids with IC50 = 6.8 μM. The quinoxaline is also able to inhibit HepG2 utilization of [14C]acetate for histone acetylation with IC50 = 5.5 μM[1].

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    MDA-MB-231-R cells

  • Concentrations

    10 uM

  • Incubation Time

    6 h

  • Method

    10 µM of ACSS2 inhibitor was added in flag-WT or KR-STAT3 expressing MDA-MB-231-R cells and incubated for 6 h.
Animal Study:

[3]
  • Animal Models

    Female C57BL/6J mice

  • Dosages

    15 mg/kg

  • Administration

    i.p.

Selleck's ACSS2 inhibitor has been cited by 21 publications

Inhibition of ACSS2-mediated histone crotonylation alleviates kidney fibrosis via IL-1β-dependent macrophage activation and tubular cell senescence [ Nat Commun, 2024, 15(1):3200] PubMed: 38615014
Brain Short-Chain Fatty Acids Induce ACSS2 to Ameliorate Depressive-Like Behavior via PPARγ-TPH2 Axis [ Research (Wash D C), 2024, 7:0400] PubMed: 38939042
Acetyl-CoA synthetase 2 induces pyroptosis and inflammation of renal epithelial tubular cells in sepsis-induced acute kidney injury by upregulating the KLF5/NF-κB pathway [ Cell Commun Signal, 2024, 22(1):187] PubMed: 38515158
Sodium benzoate induces pancreatic inflammation and β cell apoptosis partially via benzoylation [ Ecotoxicol Environ Saf, 2024, 270:115877] PubMed: 38150747
KRAS mutation-selective requirement for ACSS2 in colorectal adenoma formation [ Res Sq, 2024, rs.3.rs-3931415] PubMed: 38464238
Pyruvate metabolism controls chromatin remodeling during CD4+ T cell activation [ Cell Rep, 2023, 42(6):112583] PubMed: 37267106
Pyruvate metabolism controls chromatin remodeling during CD4+ T cell activation [ Cell Rep, 2023, 42(6):112583] PubMed: 37267106
Activation of acetyl-CoA synthetase 2 mediates kidney injury in diabetic nephropathy [ JCI Insight, 2023, 10.1172/jci.insight.165817] PubMed: 37870960
Fatty acid oxidation protects cancer cells from apoptosis by increasing mitochondrial membrane lipids [ Cell Rep, 2022, 39(9):110870] PubMed: 35649368
Crosstalk between AML and stromal cells triggers acetate secretion through the metabolic rewiring of stromal cells [ Elife, 2022, 11e75908] PubMed: 36052997

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.