Home Neuronal Signaling AChR agonist Acetylcholine iodide For research use only.

Acetylcholine iodide

Synonyms: Acetylcolina

Acetylcholine iodide (Acetylcolina) is a neurotransmitter found at neuromuscular junctions, autonomic ganglia, parasympathetic effector junctions, a subset of sympathetic effector junctions, and at many sites in the central nervous system.

Acetylcholine iodide Chemical Structure

Acetylcholine iodide Chemical Structure

CAS No. 2260-50-6

Purity & Quality Control

Batch: S471801 DMSO]54 mg/mL]false]Water]54 mg/mL]false]Ethanol]54 mg/mL]false Purity: 99.78%
99.78

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Signaling Pathway

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Biological Activity

Description Acetylcholine iodide (Acetylcolina) is a neurotransmitter found at neuromuscular junctions, autonomic ganglia, parasympathetic effector junctions, a subset of sympathetic effector junctions, and at many sites in the central nervous system.
In vitro
In vitro Acetylcholine iodide (Ach) stimulated SBC3 cell proliferation, adhesion and migration toward fibronectin (Fn) in a dose-dependent manner[1]. ACh ameliorates TNF-α-induced calpain activation by decreasing p38-MAPK phosphorylation and enhancing calpastatin expression. It elicits an anti-apoptotic effect through the activation of the muscarinic ACh receptor (MAChR) and the activation of anti-oxidant systems. ACh increases cell viability and decreases TNF-α-induced apoptosis in H9c2 cells[2].
Cell Research Cell lines The SBC3, human SCLC cell line
Concentrations 1, 10, 100 μM
Incubation Time 24, 48 or 72 h
Method The SBC3 cells are seeded at 2000 cells/well in treated 96-well plates maintained with RPMI1640 medium containing 10% (v/v) FCS for 24 h. The medium is removed and replaced with 100 μl RPMI1640 containing 1% (v/v) ITS. The cells are incubated in serum free medium for 24 h to synchronize the cell cycle. Then another 100 μl serum-free medium containing the agonist/antagonist under investigation is added. The antagonist is added 30 min before Ach(Acetylcholine iodide). At this time, the cells in a duplicate plate are given 100 μl serum-free medium and a MTT assay is used to determine live cell numbers before treatment with the agonist/antagonist as measured by the absorbance (OD) at 550 nm/650 nm. After 24, 48 or 72 h culture, the remaining cells are assayed to yield post-agonist/antagonist cell numbers using the same method. All the experiments are performed in triplicate.
In Vivo
In vivo Acetylcholine can inhibit pro-inflammatory cytokine release and protect against cardiomyocyte injury[2].
Animal Research Animal Models Female albino rats
Dosages 25 or 50 mg/kg
Administration s.c

Chemical Information & Solubility

Molecular Weight 273.11 Formula

C7H16NO2.I
CAS No. 2260-50-6 SDF Download Acetylcholine iodide SDF
Smiles CC(=O)OCC[N+](C)(C)C.[I-]
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 54 mg/mL ( (197.72 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 54 mg/mL

Ethanol : 54 mg/mL


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