Acetylcholine iodide

Catalog No.S4718 Batch:S471801

Technical Data

Formula	C₇H₁₆NO₂.I
Molecular Weight	273.11	CAS No.	2260-50-6
Solubility (25°C)*	In vitro	DMSO	54 mg/mL (197.72 mM)
		Water	54 mg/mL (197.72 mM)
		Ethanol	54 mg/mL (197.72 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Acetylcholine iodide (Acetylcolina) is a neurotransmitter found at neuromuscular junctions, autonomic ganglia, parasympathetic effector junctions, a subset of sympathetic effector junctions, and at many sites in the central nervous system.
In vitro	Acetylcholine iodide (Ach) stimulated SBC3 cell proliferation, adhesion and migration toward fibronectin (Fn) in a dose-dependent manner^[1]. ACh ameliorates TNF-α-induced calpain activation by decreasing p38-MAPK phosphorylation and enhancing calpastatin expression. It elicits an anti-apoptotic effect through the activation of the muscarinic ACh receptor (MAChR) and the activation of anti-oxidant systems. ACh increases cell viability and decreases TNF-α-induced apoptosis in H9c2 cells^[2].
In vivo	Acetylcholine can inhibit pro-inflammatory cytokine release and protect against cardiomyocyte injury^[2].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines The SBC3, human SCLC cell line Concentrations 1, 10, 100 μM Incubation Time 24, 48 or 72 h Method The SBC3 cells are seeded at 2000 cells/well in treated 96-well plates maintained with RPMI1640 medium containing 10% (v/v) FCS for 24 h. The medium is removed and replaced with 100 μl RPMI1640 containing 1% (v/v) ITS. The cells are incubated in serum free medium for 24 h to synchronize the cell cycle. Then another 100 μl serum-free medium containing the agonist/antagonist under investigation is added. The antagonist is added 30 min before Ach(Acetylcholine iodide). At this time, the cells in a duplicate plate are given 100 μl serum-free medium and a MTT assay is used to determine live cell numbers before treatment with the agonist/antagonist as measured by the absorbance (OD) at 550 nm/650 nm. After 24, 48 or 72 h culture, the remaining cells are assayed to yield post-agonist/antagonist cell numbers using the same method. All the experiments are performed in triplicate.
Animal Study:^{^[3]}	Animal Models Female albino rats Dosages 25 or 50 mg/kg Administration s.c

Cell Assay:^{^[1]}

Cell lines
The SBC3, human SCLC cell line
Concentrations
1, 10, 100 μM
Incubation Time
24, 48 or 72 h
Method
The SBC3 cells are seeded at 2000 cells/well in treated 96-well plates maintained with RPMI1640 medium containing 10% (v/v) FCS for 24 h. The medium is removed and replaced with 100 μl RPMI1640 containing 1% (v/v) ITS. The cells are incubated in serum free medium for 24 h to synchronize the cell cycle. Then another 100 μl serum-free medium containing the agonist/antagonist under investigation is added. The antagonist is added 30 min before Ach(Acetylcholine iodide). At this time, the cells in a duplicate plate are given 100 μl serum-free medium and a MTT assay is used to determine live cell numbers before treatment with the agonist/antagonist as measured by the absorbance (OD) at 550 nm/650 nm. After 24, 48 or 72 h culture, the remaining cells are assayed to yield post-agonist/antagonist cell numbers using the same method. All the experiments are performed in triplicate.

Animal Study:^{^[3]}

Animal Models
Female albino rats
Dosages
25 or 50 mg/kg
Administration
s.c

References

Selleck's Acetylcholine iodide has been cited by 2 publications

Vitamin D ameliorates asthma-induced lung injury by regulating HIF-1α/Notch1 signaling during autophagy [ Food Sci Nutr, 2022, 10(8):2773-2785]	PubMed: 35959262
Crosstalk between PKC and MAPK pathway activation in cardiac fibroblasts in a rat model of atrial fibrillation [ Biotechnol Lett, 2020, 42(7):1219-1227]	PubMed: 32095918

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SHIPPING AND STORAGE
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