Home Neuronal Signaling AChR inhibitor Lycorine For research use only.

Lycorine

Synonyms: Galanthidine, Amarylline, Narcissine, Licorine, Belamarine

Lycorine (Galanthidine, Amarylline, Narcissine, Licorine, Belamarine) is a toxic crystalline alkaloid found in various Amaryllidaceae species that weakly inhibits acetylcholinesterase (AChE) and ascorbic acid biosynthesis.

Lycorine Chemical Structure

Lycorine Chemical Structure

CAS No. 476-28-8

Purity & Quality Control

Lycorine Related Products

Signaling Pathway

AChR Signaling Pathways

AChR Signaling Pathway

Biological Activity

Description Lycorine (Galanthidine, Amarylline, Narcissine, Licorine, Belamarine) is a toxic crystalline alkaloid found in various Amaryllidaceae species that weakly inhibits acetylcholinesterase (AChE) and ascorbic acid biosynthesis.
In vitro
In vitro Lycorine can inhibit protein synthesis in eukaryotic cells and acetylcholinesterase activity. Lycorine could effectively arrest the cell cycle at the G2/M phase and induce apoptosis in HL-60 cells. It is able to block the KM3 cell cycle at G0/G1 phase through the down-regulation of both cyclin D1 and CDK4[2].
Cell Research Cell lines Caki-1; ACHN; KPK-1 and Renca cell lines
Concentrations 0, 0.5, 1, 5, and 10 μM
Incubation Time 24, 48, 72, and 96 h
Method The cell lines Caki-1; ACHN; KPK-1 and Renca are diluted in 96-well plates at a density of 1 × 103 and incubated with medium alone or serial dilutions of lycorine hydrochloride (0, 0.5, 1, 5, and 10 μM). Cell viability is monitored after 24, 48, 72, and 96 h. For each time point, the XTT labeling reagent and an electron coupling reagent is added to the culture. The cells are incubated for 4 h at 37°C and 5% CO2, and absorbance at 450–500 nm, with a reference wavelength at 650 nm, is recorded using a Bio-Rad microplate reader.
In Vivo
In vivo Lycorine, when tested in the human leukemia xenograft models, appears to exhibit anti-tumor activity in vivo. It prolongs the mean survival time of SCID leukemia xenograft models. Lycorine could alleviate the infiltration of tumor cells into tissues[2].
Animal Research Animal Models C57BL/6 mice inoculated with Renca-Luc cells into the sub-renal capsule kidney and tail vein (orthotopic tumor and metastatic lung tumors model)
Dosages 5 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 287.31 Formula

C16H17NO4
CAS No. 476-28-8 SDF --
Smiles C1CN2CC3=CC4=C(C=C3C5C2C1=CC(C5O)O)OCO4
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 40 mg/mL ( (139.22 mM) Warmed with 50°C water bath; Ultrasonicated; Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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