Lycorine

Catalog No.S3903 Batch:S390304

Technical Data

Formula	C₁₆H₁₇NO₄
Molecular Weight	287.31	CAS No.	476-28-8
Solubility (25°C)*	In vitro	DMSO	40 mg/mL (139.22 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Lycorine (Galanthidine, Amarylline, Narcissine, Licorine, Belamarine) is a toxic crystalline alkaloid found in various Amaryllidaceae species that weakly inhibits acetylcholinesterase (AChE) and ascorbic acid biosynthesis.
In vitro	Lycorine can inhibit protein synthesis in eukaryotic cells and acetylcholinesterase activity. Lycorine could effectively arrest the cell cycle at the G2/M phase and induce apoptosis in HL-60 cells. It is able to block the KM3 cell cycle at G0/G1 phase through the down-regulation of both cyclin D1 and CDK4^[2].
In vivo	Lycorine, when tested in the human leukemia xenograft models, appears to exhibit anti-tumor activity in vivo. It prolongs the mean survival time of SCID leukemia xenograft models. Lycorine could alleviate the infiltration of tumor cells into tissues^[2].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines Caki-1; ACHN; KPK-1 and Renca cell lines Concentrations 0, 0.5, 1, 5, and 10 μM Incubation Time 24, 48, 72, and 96 h Method The cell lines Caki-1; ACHN; KPK-1 and Renca are diluted in 96-well plates at a density of 1 × 10³ and incubated with medium alone or serial dilutions of lycorine hydrochloride (0, 0.5, 1, 5, and 10 μM). Cell viability is monitored after 24, 48, 72, and 96 h. For each time point, the XTT labeling reagent and an electron coupling reagent is added to the culture. The cells are incubated for 4 h at 37°C and 5% CO2, and absorbance at 450–500 nm, with a reference wavelength at 650 nm, is recorded using a Bio-Rad microplate reader.
Animal Study:^{^[1]}	Animal Models C57BL/6 mice inoculated with Renca-Luc cells into the sub-renal capsule kidney and tail vein (orthotopic tumor and metastatic lung tumors model) Dosages 5 mg/kg Administration i.p.

Cell Assay:^{^[1]}

Cell lines
Caki-1; ACHN; KPK-1 and Renca cell lines
Concentrations
0, 0.5, 1, 5, and 10 μM
Incubation Time
24, 48, 72, and 96 h
Method
The cell lines Caki-1; ACHN; KPK-1 and Renca are diluted in 96-well plates at a density of 1 × 10³ and incubated with medium alone or serial dilutions of lycorine hydrochloride (0, 0.5, 1, 5, and 10 μM). Cell viability is monitored after 24, 48, 72, and 96 h. For each time point, the XTT labeling reagent and an electron coupling reagent is added to the culture. The cells are incubated for 4 h at 37°C and 5% CO2, and absorbance at 450–500 nm, with a reference wavelength at 650 nm, is recorded using a Bio-Rad microplate reader.

Animal Study:^{^[1]}

Animal Models
C57BL/6 mice inoculated with Renca-Luc cells into the sub-renal capsule kidney and tail vein (orthotopic tumor and metastatic lung tumors model)
Dosages
5 mg/kg
Administration
i.p.

References

Selleck's Lycorine has been cited by 2 publications

Lycorine weakens tamoxifen resistance of breast cancer via abrogating HAGLR-mediated epigenetic suppression on VGLL4 by DNMT1 [ Kaohsiung J Med Sci, 2023, none]	PubMed: 36606584
A multi-targeting drug design strategy for identifying potent anti-SARS-CoV-2 inhibitors [ Acta Pharmacol Sin, 2021, 1-11]	PubMed: 33907306

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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