Home Microbiology Antibiotics inhibitor Methacycline HCl For research use only.

Methacycline HCl

Synonyms: Rondomycin

Methacycline HCl (Rondomycin) is a tetracycline antibiotic, and also an inhibitor of epithelial–mesenchymal transition (EMT) with IC50 of roughly 5 μM, used to treat various infections.

Methacycline HCl Chemical Structure

Methacycline HCl Chemical Structure

CAS No. 3963-95-9

Purity & Quality Control

Methacycline HCl Related Products

Biological Activity

Description Methacycline HCl (Rondomycin) is a tetracycline antibiotic, and also an inhibitor of epithelial–mesenchymal transition (EMT) with IC50 of roughly 5 μM, used to treat various infections.
Targets
protein synthesis [1]
In vitro
In vitro Methacycline inhibits TGF-β1-induced α-smooth muscle actin, Snail1, and collagen I of primary alveolar epithelial cells. Methacycline inhibits TGF-β1-induced non-Smad pathways, including c-Jun N-terminal kinase, p38, and Akt activation, but not Smad or β-catenin transcriptional activity. Methacycline has no effect on baseline c-Jun N-terminal kinase, p38, or Akt activities or lung fibroblast responses to TGF-β1.[1]
In Vivo
In vivo Methacycline (100 mg/kg, i.p.) delivered daily beginning 10 days after intratracheal bleomycin improves survival at Day 17. Methacycline attenuates Bleomycin-induced canonical EMT markers, Snail1, Twist1, collagen I, as well as fibronectin protein and mRNA. Methacycline does not attenuate inflammatory cell accumulation or alter TGF-β1-responsive genes in alveolar macrophages. [1]

Chemical Information & Solubility

Molecular Weight 478.88 Formula

C22H22N2O8.HCl
CAS No. 3963-95-9 SDF Download Methacycline HCl SDF
Smiles CN(C)C1C2C(C3C(=C)C4=C(C(=CC=C4)O)C(=C3C(=O)C2(C(=C(C1=O)C(=O)N)O)O)O)O.Cl
Storage (From the date of receipt)

In vitro
Batch:

Water : 5 mg/mL

DMSO : 3 mg/mL ( (6.26 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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