Home Neuronal Signaling COX inhibitor Asaraldehyde For research use only.

Asaraldehyde

Synonyms: Asaronaldehyde

Asaraldehyde (Asaronaldehyde) is a natural COX-2 inhibitor, exhibiting 17-fold selectivity over COX-1.

Asaraldehyde Chemical Structure

Asaraldehyde Chemical Structure

CAS No. 4460-86-0

Purity & Quality Control

Batch: S253101 DMSO]39 mg/mL]false]Ethanol]16 mg/mL]false]Water]Insoluble]false Purity: 100%
100

Asaraldehyde Related Products

Signaling Pathway

COX Signaling Pathways

COX Signaling Pathway

Biological Activity

Description Asaraldehyde (Asaronaldehyde) is a natural COX-2 inhibitor, exhibiting 17-fold selectivity over COX-1.
Targets
COX-2 [2]
In vitro
In vitro Asaraldehyde is found to be the most abundant constituent, but is totally absent in its cultured broth and its natural host, C. kanehirae wood. On feeding with the acid-digested sawdust of C. kanehirae wood or vanillin to the broth for culture, Asaraldehyde is produced in both cultured broths. [1] Asaraldehyde shows 3.32% of prostaglandin H endoperoxide synthase-1 (COX-1) inhibitory activity and 52.69% prostaglandin H endoperoxide synthase-2 (COX-2) inhibitory activity, respectively at 100 mg/mL. Asaraldehyde shows selectivity towards COX-2 enzyme inhibition at 100 µg/mL. The COX-2/COX-1 ratio for Asaraldehyde is 17.68 at 100 µg/mL compared to solvent control. [2] Asaraldehyde down-regulates C/EBPβ, C/EBPδ, and C/EBPα. Asaraldehyde suppresses expression of PPARγ1 and PPARγ2. Asaraldehyde also significantly suppresses the expression of acetyl-CoA carboxylase (ACC). [2]
Cell Research Cell lines 3T3-L1 cells
Concentrations 100 μg/mL
Incubation Time 24 hours
Method 3T3-L1 cells are seeded in 96-well plates at a concentration of 104 /well. Twenty-four hours after seeding, the cells are treated with 100 μg/mL of Asaraldehyde for 24 hours or for the whole 8-day differentiation period. Fully differentiated adipocytes are also treated with 100 μg/mL of Asaraldehyde for 24 hours-72 hours to test the cytotoxicity. At the end of treatment, cells are cultured with MTT at a final concentration of 0.5 mg/mL for another 4 hours. The purple MTT formazan is dissolved by DMSO and the absorbance at 570 nm is taken with a spectrophotometer. The absorbance is proportional to the viability of adipocytes.

Chemical Information & Solubility

Molecular Weight 196.2 Formula

C10H12O4
CAS No. 4460-86-0 SDF Download Asaraldehyde SDF
Smiles COC1=CC(=C(C=C1C=O)OC)OC
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 39 mg/mL ( (198.77 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 16 mg/mL

Water : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Asaraldehyde | Asaraldehyde supplier | purchase Asaraldehyde | Asaraldehyde cost | Asaraldehyde manufacturer | order Asaraldehyde | Asaraldehyde distributor