Home Metabolism Carbonic Anhydrase inhibitor U-104 For research use only.

U-104

Synonyms: MST-104, NSC 213841, SLC-0111, WBI-5111

U-104 (MST-104, NSC 213841, SLC-0111, WBI-5111) is a potent carbonic anhydrase (CA) inhibitor for CA IX and CA XII with Ki of 45.1 nM and 4.5 nM, respectively, very low inhibition for CA I and CA II.

U-104 Chemical Structure

U-104 Chemical Structure

CAS No. 178606-66-1

Purity & Quality Control

U-104 Related Products

Signaling Pathway

Carbonic Anhydrase Signaling Pathways

Carbonic Anhydrase Signaling Pathway

Biological Activity

Description U-104 (MST-104, NSC 213841, SLC-0111, WBI-5111) is a potent carbonic anhydrase (CA) inhibitor for CA IX and CA XII with Ki of 45.1 nM and 4.5 nM, respectively, very low inhibition for CA I and CA II.
Targets
CAXII [1]
(Cell-free assay)		 CAIX [1]
(Cell-free assay)
4.5 nM(Ki) 45.1 nM(Ki)
In vitro
In vitro U-104 (50  μM) blocks the mesenchymal phenotype in the cancer stem cells population in hypoxia condition of 4T1 cells. U-104 (<50  μM) significantly reduces migration in a dose-dependent manner in metastatic MDA-MB-231 LM2-4Luc+ cells , with cells growing as compact colonies similar to parental MDA-MB-231 cells. [2] U-104 possesses aromatic groups at the second nitrogen ureido group. [3]
In Vivo
In vivo U-104 (38 mg/kg) inhibits primary tumor growth in the mice implanted orthotopically with MDA-MB-231 LM2-4Luc+ cells. U-104 (19 mg/kg) inhibits metastases formation in the 4T1 experimental metastasis mice model. [1] U-104 (38 mg/kg) significantly delay primary tumor growth and reduces cancer stem cell population in NOD/SCID mice orthotopically implanted with MDA-MB-231 LM2-4Luc+ cells. U-104 (5 mg/mL, oral gavage) shows a significant delay in tumor growth in Balb/c mice orthotopically implanted with 4T1 cells. [2]
Animal Research Animal Models Balb/c mice orthotopically implanted with 4T1 cells.
Dosages 5 mg/mL
Administration Oral gavage
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03450018 Recruiting
Metastatic Pancreatic Ductal Adenocarcinoma
British Columbia Cancer Agency|Canadian Cancer Society (CCS)|SignalChem Lifesciences Corporation
 January 10 2019 Phase 1|Phase 2
NCT02215850 Completed
Solid Tumours
Welichem Biotech Inc.|Ozmosis Research Inc.
 October 2014 Phase 1

Chemical Information & Solubility

Molecular Weight 309.32 Formula

C13H12FN3O3S
CAS No. 178606-66-1 SDF Download U-104 SDF
Smiles C1=CC(=CC=C1NC(=O)NC2=CC=C(C=C2)S(=O)(=O)N)F
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 62 mg/mL ( (200.43 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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