Tocilizumab (anti-IL-6R)

Synonyms: MRA

Catalog No.A2012 Purity:99%

Tocilizumab (anti-IL-6R) is a humanized monoclonal antibody that binds to the interleukin-6 receptor, MW: 148 KD.

Tocilizumab (anti-IL-6R) Chemical Structure

Click to purchase the isotype control of Tocilizumab (anti-IL-6R)

Selleck's Tocilizumab (anti-IL-6R) has been cited by 44 publications

Purity & Quality Control

Batch: Purity: 99% Protein concentration: 14.29mg/ml Endotoxin Level: ＜1EU/mg

Choose Selective IL Receptor Inhibitors

Expand to Check More

1. "+" indicates inhibitory effect. Increased inhibition is marked by a higher "+" designation. 2. "✔" indicates inhibitory effect, but without specific value.

Biological Activity

Description

Tocilizumab (anti-IL-6R) is a humanized monoclonal antibody that binds to the interleukin-6 receptor, MW: 148 KD.

Features

Human IgG1

Targets

IL-6R ^[1]

In Vitro
In vitro	After adding tocilizumab at different concentrations into HMO6 and CHME5 cells stimulated by F3-CM or with IL6, the expression levels of anti-inflammatory M2 markers (CD206 and Arg1) are decreased, as well as the levels of both phosphorylated JAK2 and STAT3.^[1]
Cell Research:	HMO6 and CHME5 cells with control media or F3-CM alone or F3-CM were treated with Tocilizumab (2.5 μg/ml for 24 h) or with IL6 (200 ng/ml for 24 h) alone or IL6-supplemented with Tocilizumab. Western blot analysis of p-JAK2, JAK2, p-STAT3, and STAT3 in HMO6 and CHME5 cells were conducted.

In Vitro

In vitro

After adding tocilizumab at different concentrations into HMO6 and CHME5 cells stimulated by F3-CM or with IL6, the expression levels of anti-inflammatory M2 markers (CD206 and Arg1) are decreased, as well as the levels of both phosphorylated JAK2 and STAT3.^[1]

Cell Research:

HMO6 and CHME5 cells with control media or F3-CM alone or F3-CM were treated with Tocilizumab (2.5 μg/ml for 24 h) or with IL6 (200 ng/ml for 24 h) alone or IL6-supplemented with Tocilizumab. Western blot analysis of p-JAK2, JAK2, p-STAT3, and STAT3 in HMO6 and CHME5 cells were conducted.

In Vivo
In vivo	Tocilizumab can block JAK2/STAT3 activation and impede brain metastasis (BM) of A549-F3 cells in vivo.^[1]
Animal Research	Animal Models: Nude mice of brain metastasis models Dosages: 5 mg/kg Administration: i.p. Reference: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8864012/

References
[1] Jin Y, et al. Signal Transduct Target Ther. 2022 Feb 23;7(1):52.

References

[1] Jin Y, et al. Signal Transduct Target Ther. 2022 Feb 23;7(1):52.

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

Product Details

CAS No.	‎375823-41-9
Isotype	Human IgG1
Source	CHO cells
Formulation	PBS buffer, pH 7.2
Storage (From the date of receipt)	Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles

Comparison of Clones for

^*Literature analysis of various clone (for this target) products available on the market shows that Selleck's selected clones are more frequently applied. (data until September 2024)

Tech Support

If you have any other enquiries, please leave a message.

* Indicates a Required Field

* Name Please enter your name.

* Email Please enter your email. Please enter a valid email address.

Phone

* Comments: Please write something to us.

Frequently Asked Questions

Question 1:
How to store the antibody?

Answer:
Store the undiluted solution at 4 °C in the dark. Freezing antibodies can result in a loss of activity caused by the freezing/thawing process. Diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided. Additionally, make sure to keep the antibody sterile. Under these storage conditions, your antibodies should remain active for up to one year and oftentimes longer.