Tocilizumab (anti-IL-6R)

Catalog No.A2012        Batch: A201202

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Technical Data

CAS No. ‎375823-41-9
Formulation PBS buffer, pH 7.2
Isotype Human IgG1
Source CHO cells
Storage
(From the date of receipt)		 Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles
Purity 98.85%
Protein concentration 4.97mg/ml
Endotoxin Level ≤1 EU/mg

Biological Activity

Description Tocilizumab (anti-IL-6R) is a humanized monoclonal antibody that binds to the interleukin-6 receptor, MW: 148 KD.
Targets
IL-6R [1]
In vitro

After adding tocilizumab at different concentrations into HMO6 and CHME5 cells stimulated by F3-CM or with IL6, the expression levels of anti-inflammatory M2 markers (CD206 and Arg1) are decreased, as well as the levels of both phosphorylated JAK2 and STAT3.[1]
In vivo

Tocilizumab can block JAK2/STAT3 activation and impede brain metastasis (BM) of A549-F3 cells in vivo.[1]

Protocol (Only for Reference)

Cell Assay:

  • HMO6 and CHME5 cells with control media or F3-CM alone or F3-CM were treated with Tocilizumab (2.5 μg/ml for 24 h) or with IL6 (200 ng/ml for 24 h) alone or IL6-supplemented with Tocilizumab. Western blot analysis of p-JAK2, JAK2, p-STAT3, and STAT3 in HMO6 and CHME5 cells were conducted.
Animal Study:

  • Animal Models: Nude mice of brain metastasis models
    Dosages: 5 mg/kg
    Administration: i.p.
    Reference: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8864012/
     

Selleck's Tocilizumab (anti-IL-6R) has been cited by 50 publications

AKT and EZH2 inhibitors kill TNBCs by hijacking mechanisms of involution [ Nature, 2024, 10.1038/s41586-024-08031-6] PubMed: 39385030
Development of a chimeric cytokine receptor that captures IL-6 and enhances the antitumor response of CAR-T cells [ Cell Rep Med, 2024, S2666-3791(24)00195-2] PubMed: 38670095
CALB2 drives pancreatic cancer metastasis through inflammatory reprogramming of the tumor microenvironment [ J Exp Clin Cancer Res, 2024, 43(1):277] PubMed: 39358777
Cells in the Polyaneuploid Cancer Cell State are Pro-Metastatic [ bioRxiv, 2024, 2024.07.12.603285] PubMed: 39071340
Autoimmunity in Down's syndrome via cytokines, CD4 T cells and CD11c+ B cells [ Nature, 2023, 615(7951):305-314] PubMed: 36813963
Autoimmunity in Down's syndrome via cytokines, CD4 T cells and CD11c+ B cells [ Nature, 2023, 615(7951):305-314] PubMed: 36813963
Autoimmunity in Down's syndrome via cytokines, CD4 T cells and CD11c+ B cells [ Nature, 2023, 615(7951):305-314] PubMed: 36813963
Autoimmunity in Down's syndrome via cytokines, CD4 T cells and CD11c+ B cells [ Nature, 2023, 615(7951):305-314] PubMed: 36813963
Peripheral helper-T-cell-derived CXCL13 is a crucial pathogenic factor in idiopathic multicentric Castleman disease [ Nat Commun, 2023, 14(1):6959] PubMed: 37907518
Anti-inflammatory effect of interleukin-6 highly enriched in secretome of two clinically relevant sources of mesenchymal stromal cells [ Front Cell Dev Biol, 2023, 11:1244120] PubMed: 37745306

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

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