Home Metabolism GLUT inhibitor STF-31 For research use only.

STF-31

STF-31 is a selective glucose transporter GLUT1 inhibitor.

STF-31 Chemical Structure

STF-31 Chemical Structure

CAS No. 724741-75-7

Purity & Quality Control

STF-31 Related Products

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A2780 cells Function assay 48 h Inhibition of NAMPT in human A2780 cells assessed as reduction in NAD level after 48 hrs by LC-MS/MS analysis, IC50=0.024 μM 24433859
RCC4 cells Cytotoxicity assay 4 days Cytotoxicity against human RCC4 cells after 4 days by XTT assay, IC50=0.16 μM 24387866
HT1080 cells Proliferation assay 96 h Antiproliferative activity against human HT1080 cells after 96 hrs by CyQuant-based assay, IC50=0.213 μM 24433859
PC3 cells Proliferation assay 96 h Antiproliferative activity against human PC3 cells after 96 hrs by CyQuant-based assay, IC50=0.217 μM 24433859
HCT116 cells Proliferation assay 96 h Antiproliferative activity against human HCT116 cells after 96 hrs by CyQuant-based assay, IC50=0.271 μM 24433859
MIAPaCa2 cells Proliferation assay 96 h Antiproliferative activity against human MIAPaCa2 cells after 96 hrs by CyQuant-based assay, IC50=0.855 μM 24433859
Click to View More Cell Line Experimental Data

Biological Activity

Description STF-31 is a selective glucose transporter GLUT1 inhibitor.
Targets
GLUT1 [1]
In vitro
In vitro STF-31 selectively kills RCCs by specifically targeting glucose uptake through GLUT1. STF-31 significantly inhibits lactate production and extracellular acidification in VHL-deficient cells by about 60%, and decreases glycolysis by decreasing glucose transport. [1] In NAPRT1-expressing cells, STF-31 shows cytotoxicity by inhibiting the enzymatic activity of NAMPT. [2] STF-31 is toxic to hPSCs and efficient for selective elimination of hPSCs from mixed cultures. [3]
Cell Research Cell lines RCC4/VHL and RCC4 cells
Concentrations --
Incubation Time 4 d
Method

For XTT assays, five thousand cells are plated in 96-well plates. The next day, vehicle (DMSO) or drug is added by serial dilution. Four days later, media are aspirated, XTT solution (0.3 mg/ml of XTT, 2.65 μg/ml N-methyl dibenxopyrazine methyl sulfate in phenol red-free media) is added, and the plates are incubated at 37°C for 1-2 hours. Metabolism of XTT is quantified by measuring the absorbance at 450 nm. IC50s are calculated using linear interpolation. All conditions are measured in triplicate and each experiment is done in duplicate or triplicate.
Experimental Result Images Methods Biomarkers Images PMID
Western blot TFCP2 / ASIC2a p-EGFR / HIF-1α / c-Myc / Glut-1 28725010
Immunofluorescence ASIC2a / TFCP2 28725010
In Vivo
In vivo In mice with VHL-deficient RCC xenografts, a more soluble analog of STF-31 (11.6 mg/kg, i.p.) markedly delays tumor growth. [1]
Animal Research Animal Models Mice with VHL-deficient RCC xenografts
Dosages 11.6 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 423.53 Formula

C23H25N3O3S
CAS No. 724741-75-7 SDF Download STF-31 SDF
Smiles CC(C)(C)C1=CC=C(C=C1)S(=O)(=O)NCC2=CC=C(C=C2)C(=O)NC3=CN=CC=C3
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 85 mg/mL ( (200.69 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 25 mg/mL

Water : Insoluble


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In vivo
Batch:

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In vivo Formulation Calculator

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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