STF-31

Catalog No.S7931 Batch:S793101

Technical Data

Formula	C₂₃H₂₅N₃O₃S
Molecular Weight	423.53	CAS No.	724741-75-7
Solubility (25°C)*	In vitro	DMSO	85 mg/mL (200.69 mM)
		Ethanol	25 mg/mL (59.02 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

STF-31 is a selective glucose transporter GLUT1 inhibitor.

Targets

GLUT1 ^[1]

In vitro

STF-31 selectively kills RCCs by specifically targeting glucose uptake through GLUT1. STF-31 significantly inhibits lactate production and extracellular acidification in VHL-deficient cells by about 60%, and decreases glycolysis by decreasing glucose transport. ^[1] In NAPRT1-expressing cells, STF-31 shows cytotoxicity by inhibiting the enzymatic activity of NAMPT. ^[2] STF-31 is toxic to hPSCs and efficient for selective elimination of hPSCs from mixed cultures. ^[3]

In vivo

In mice with VHL-deficient RCC xenografts, a more soluble analog of STF-31 (11.6 mg/kg, i.p.) markedly delays tumor growth. ^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines RCC4/VHL and RCC4 cells Concentrations -- Incubation Time 4 d Method For XTT assays, five thousand cells are plated in 96-well plates. The next day, vehicle (DMSO) or drug is added by serial dilution. Four days later, media are aspirated, XTT solution (0.3 mg/ml of XTT, 2.65 μg/ml N-methyl dibenxopyrazine methyl sulfate in phenol red-free media) is added, and the plates are incubated at 37°C for 1-2 hours. Metabolism of XTT is quantified by measuring the absorbance at 450 nm. IC50s are calculated using linear interpolation. All conditions are measured in triplicate and each experiment is done in duplicate or triplicate.
Animal Study:^{^[1]}	Animal Models Mice with VHL-deficient RCC xenografts Dosages 11.6 mg/kg Administration i.p.

Cell Assay:^{^[1]}

Cell lines
RCC4/VHL and RCC4 cells
Concentrations
--
Incubation Time
4 d
Method
For XTT assays, five thousand cells are plated in 96-well plates. The next day, vehicle (DMSO) or drug is added by serial dilution. Four days later, media are aspirated, XTT solution (0.3 mg/ml of XTT, 2.65 μg/ml N-methyl dibenxopyrazine methyl sulfate in phenol red-free media) is added, and the plates are incubated at 37°C for 1-2 hours. Metabolism of XTT is quantified by measuring the absorbance at 450 nm. IC50s are calculated using linear interpolation. All conditions are measured in triplicate and each experiment is done in duplicate or triplicate.

Animal Study:^{^[1]}

Animal Models
Mice with VHL-deficient RCC xenografts
Dosages
11.6 mg/kg
Administration
i.p.

References

Customer Product Validation

: Data from [Data independently produced by , , J Virol, 2017, 91(6)]

Selleck's STF-31 has been cited by 12 publications

Disruption of the IL-33-ST2-AKT signaling axis impairs neurodevelopment by inhibiting microglial metabolic adaptation and phagocytic function [ Immunity, 2022, 55(1):159-173.e9]	PubMed: 34982959
Core-shell nanosystems designed for effective oral delivery of polypeptide drugs [ J Control Release, 2022, 352:540-555]	PubMed: 36323363
Targeted inhibition of STAT3 induces immunogenic cell death of hepatocellular carcinoma cells via glycolysis [ Mol Oncol, 2022, 16(15):2861-2880]	PubMed: 35665592
A positive feedback circuit comprising p21 and HIF-1α aggravates hypoxia-induced radioresistance of glioblastoma by promoting Glut1/LDHA-mediated glycolysis [ FASEB J, 2022, 36(3):e22229]	PubMed: 35199870
Bacteria Boost Mammalian Host NAD Metabolism by Engaging the Deamidated Biosynthesis Pathway. [ Cell Metab, 2020, 31(3):564-579]	PubMed: 32130883
Marizomib suppresses triple-negative breast cancer via proteasome and oxidative phosphorylation inhibition. [ Theranostics, 2020, 6;10(12):5259-5275]	PubMed: 32373211
Improved Immunoregulation of Ultra-Low-Dose Silver Nanoparticle-Loaded TiO2 Nanotubes via M2 Macrophage Polarization by Regulating GLUT1 and Autophagy. [ Int J Nanomedicine, 2020, 24;15:2011-2026]	PubMed: 32273699
Activating Transcription Factor 3 Coordinates Differentiation of Cardiac and Hematopoietic Progenitors by Regulating Glucose Metabolism [ Sci Adv, 2020, 6;6(19):eaay9466]	PubMed: 32494702
mTORC2-mediated PDHE1α nuclear translocation links EBV-LMP1 reprogrammed glucose metabolism to cancer metastasis in nasopharyngeal carcinoma. [ Oncogene, 2019, 38(24):4669-4684]	PubMed: 30745576
Monosaccharide Analogues of Anticancer Peptide R-Lycosin-I: Role of Monosaccharide Conjugation in Complexation and the Potential of Lung Cancer Targeting and Therapy. [ J Med Chem, 2019, 62(17):7857-7873]	PubMed: 31276399

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SHIPPING AND STORAGE
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