Home Stem Cells & Wnt Hedgehog/Smoothened agonist SAG (Smoothened Agonist) For research use only.

SAG (Smoothened Agonist)

SAG (Smoothened Agonist) is a potent agonist of Smoothened (Smo). Smoothened Agonist induces firefly luciferase expression in Shh-LIGHT2 cells with an EC50 of 3 nM.

SAG (Smoothened Agonist) Chemical Structure

SAG (Smoothened Agonist) Chemical Structure

CAS No. 912545-86-9

Purity & Quality Control

SAG (Smoothened Agonist) Related Products

Signaling Pathway

Hedgehog/Smoothened Signaling Pathways

Hedgehog/Smoothened Signaling Pathway

Biological Activity

Description SAG (Smoothened Agonist) is a potent agonist of Smoothened (Smo). Smoothened Agonist induces firefly luciferase expression in Shh-LIGHT2 cells with an EC50 of 3 nM.
Targets
Smo [1]
(in Shh-LIGHT2 cells)
3 nM(EC50)
In vitro
In vitro

SAG can counteract glucocorticoids (GCs) antagonism of Shh-driven granule neuron precursor (CGNP) proliferation.[2]
Cell Research Cell lines Granule neuron precursor (CGNP) primary cells
Concentrations 15-240 nM
Incubation Time 24 h
Method

CGNP are stimulated by various SAG concentrations (15 to 240 nM) compared with ShhN (3 μg/ml) and vehicle after 24 hours in vitro. Quantitative reverse transcription–PCR is performed with SYBR Green master mix in LightCycler 480.
In Vivo
In vivo

Systemic administration of Smoothened Agonist (SAG) prevents the neurotoxic effects of glucocorticoids. Smoothened Agonist can cross the blood-brain barrier to activate Shh transcriptional targets in vivo. SAG at the treatment dose effectively prevents GC-induced neonatal cerebellar developmental abnormalities.[2]
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05937178 Recruiting
Hepatitis B Chronic
Huashan Hospital|The First Affiliated Hospital of Anhui Medical University|Anhui Provincial Hospital|Beijing YouAn Hospital|Peking University People''s Hospital|Peking University First Hospital|Xiamen Hospital of Traditional Chinese Medicine|First Affiliated Hospital of Fujian Medical University|LanZhou University|Third Affiliated Hospital Sun Yat-Sen University|First Affiliated Hospital of Guangxi Medical University|The Affiliated Hospital Of Guizhou Medical University|Hainan General Hospital|Hebei Medical University Third Hospital|The First Affiliated Hospital of Henan University of Traditional Chinese Medicine|Henan Provincial People''s Hospital|The Second Affiliated Hospital of Harbin Medical University|Tongji Hospital|Wuhan Union Hospital China|Renmin Hospital of Wuhan University|Central South University|Xiangya Hospital of Central South University|The First Hospital of Jilin University|the Second Hospital of Nangjing|The Affiliated Nanjing Drum Tower Hospital of Nanjing University Medical School|The First Affiliated Hospital with Nanjing Medical University|The Affiliated Hospital of Xuzhou Medical University|The First Affiliated Hospital of Nanchang University|Shengjing Hospital|Qingdao Sixth People''s Hospital|Shandong Provincial Hospital|The Second Hospital of Shandong University|The First Affiliated Hospital of Shanxi Medical University|Tang-Du Hospital|First Affiliated Hospital Xi''an Jiaotong University|Ruijin Hospital|West China Hospital|Tianjin Second People''s Hospital|First Affiliated Hospital of Xinjiang Medical University|The First People''s Hospital of Yunnan|Shulan (Hangzhou) Hospital|Zhejiang University|Southwest Hospital China|The Second Affiliated Hospital of Chongqing Medical University|Sichuan Provincial People''s Hospital
 January 31 2023 --
NCT05701592 Recruiting
Posterior Cruciate Ligament Tear
Konrad Malinowski MD|Artromedical Konrad Malinowski Clinic
 December 20 2022 --
NCT03645044 Active not recruiting
Hiv|HBV Coinfection
University of Melbourne|National Health and Medical Research Council Australia|The University of Western Australia|University of Adelaide|The HIV Netherlands Australia Thailand Research Collaboration|University of Malaya|YR Gaitonde Centre for AIDS Research and Education|Melbourne Health
 May 24 2018 --

Chemical Information & Solubility

Molecular Weight 490.06 Formula

C28H28ClN3OS
CAS No. 912545-86-9 SDF --
Smiles CNC1CCC(CC1)N(CC2=CC(=CC=C2)C3=CC=NC=C3)C(=O)C4=C(Cl)C5=CC=CC=C5S4
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 98 mg/mL ( (199.97 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 98 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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