Home GPCR & G Protein Hedgehog/Smoothened inhibitor Ciliobrevin D For research use only.

Ciliobrevin D

Ciliobrevin D (compound 5) is a cell-permeable, reversible and specific antagonist of AAA+ (ATPases associated with diverse cellular activities) ATPase motor cytoplasmic dynein. Ciliobrevin D perturbs primary cilia formation and blocks Hedgehog (Hh) signaling.

Ciliobrevin D Chemical Structure

Ciliobrevin D Chemical Structure

CAS No. 1370554-01-0

Purity & Quality Control

Batch: S974301 DMSO]20 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.77%
99.77

Ciliobrevin D Related Products

Signaling Pathway

Hedgehog/Smoothened Signaling Pathways

Hedgehog/Smoothened Signaling Pathway

Biological Activity

Description Ciliobrevin D (compound 5) is a cell-permeable, reversible and specific antagonist of AAA+ (ATPases associated with diverse cellular activities) ATPase motor cytoplasmic dynein. Ciliobrevin D perturbs primary cilia formation and blocks Hedgehog (Hh) signaling.
Targets
cytoplasmic dynein [1] Hedgehog [1]
In vitro
In vitro

Ciliobrevin D inactivate dynein in Sertoli cells. The inactivation of dynein by ciliobrevin D also perturbs gross disruption of F-actin across the Sertoli cells in vitro.[2] Ciliobrevin D perturbs protein trafficking within the primary cilium, leading to their malformation and Hedgehog signaling blockade. Ciliobrevin D also prevents spindle pole focusing, kinetochore-microtubule attachment, melanosome aggregation, and peroxisome motility in cultured cells.[1]
Cell Research Cell lines Sertoli cells
Concentrations 15 µM, 30 µM
Incubation Time 1 h
Method

Sertoli cells cultured in vitro on Matrigel-coated bicameral units at 1.0 × 106 cells/cm2 are used for quantifying the transepithelial electrical resistance in ohms (Ω) across the cell epithelium to assess the TJ-barrier function. In brief, each bicameral unit is placed inside the well of a 24-well dish with 0.5 ml F12/DMEM in the apical and the basal compartments. Dynein is inhibited in Sertoli cells on days 3 and 4 using Ciliobrevin D at 15 µM or 30 µM for 1hr, and Sertoli cell TJ-permeability barrier function is monitored daily by quantifying transepithelial electrical resistance across the cell epithelium.
In Vivo
In vivo

Ciliobrevin D inactivate dynein in the testis. The inactivation of dynein by ciliobrevin D also perturbs gross disruption of F-actin across the seminiferous epithelium in vivo, illustrating there are cross talks between the two cytoskeletons in the testis.[2]
Animal Research Animal Models adult male Sprague-Dawley rats
Dosages 15 µM per testis
Administration local injection

Chemical Information & Solubility

Molecular Weight 392.62 Formula

C17H8Cl3N3O2
CAS No. 1370554-01-0 SDF --
Smiles ClC1=CC(=C(C=C1)C(=O)C(\C#N)=C2/NC(=O)C3=CC=C(Cl)C=C3N2)Cl
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 20 mg/mL ( (50.93 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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