Home Neuronal Signaling Notch inhibitor RIN1 (RBPJ Inhibitor-1) For research use only.

RIN1 (RBPJ Inhibitor-1)

RIN1 (RBPJ Inhibitor-1) is a potent inhibitor of the transcription factor RBPJ that disrupts the interaction between NOTCH and RBPJ.

RIN1 (RBPJ Inhibitor-1) Chemical Structure

RIN1 (RBPJ Inhibitor-1) Chemical Structure

CAS No. 2682114-39-0

Purity & Quality Control

RIN1 (RBPJ Inhibitor-1) Related Products

Biological Activity

Description RIN1 (RBPJ Inhibitor-1) is a potent inhibitor of the transcription factor RBPJ that disrupts the interaction between NOTCH and RBPJ.
Targets
RBPJ [1]
In vitro
In vitro

RBPJ Inhibitor-1 (RIN1) blocks the functional interaction of RBPJ with SHARP, a scaffold protein that forms a transcriptional repressor complex with RBPJ in the absence of NOTCH signaling. Consistent with disruption of NOTCH signaling, RIN1 inhibits the proliferation of hematologic cancer cell lines and promotes skeletal muscle differentiation from C2C12 myoblasts.[1]
Cell Research Cell lines AD-293 cells
Concentrations 2 µM
Incubation Time 17 h
Method

AD-293 cells were transfected with RBPJ-VP16myc and 48 hours later were treated±RIN1 (2 µM), Cycloheximide (CHX, 10 µg/ml) for an additional 17 hours and then assayed for Hes1-Luciferase activity.
In Vivo
In vivo

RBPJ Inhibitor-1 (RIN1) is a potent inhibitor of the transcription factor RBPJ that disrupts the interaction between NOTCH and RBPJ.

Chemical Information & Solubility

Molecular Weight 311.31 Formula

C17H14FN3O2
CAS No. 2682114-39-0 SDF --
Smiles C[N]1N=CC=C1C2=CC(=C(C=C2)C(N)=O)OC3=CC=CC=C3F
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 62 mg/mL ( (199.15 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 62 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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