Home Microbiology Parasite inhibitor Rafoxanide For research use only.

Rafoxanide

Synonyms: Disalan, Ranide, Flukanide, Bovanide

Rafoxanide (Disalan, Ranide, Flukanide, Bovanide) is a salicylanilide used as an anthelmintic, being effective treatment for the control of fluke infections in animals.

Rafoxanide Chemical Structure

Rafoxanide Chemical Structure

CAS No. 22662-39-1

Purity & Quality Control

Batch: S509301 DMSO]100 mg/mL]false]]]false]]]false Purity: 98%
98

Rafoxanide Related Products

Biological Activity

Description Rafoxanide (Disalan, Ranide, Flukanide, Bovanide) is a salicylanilide used as an anthelmintic, being effective treatment for the control of fluke infections in animals.
In vitro
In vitro Rafoxanide is a novel allosteric inhibitor of SPAK and OSR1. In vitro kinase assays using Rafoxanide showed it to be a low micromolar inhibitor of OSR1 T185E in vitro, IC50 = 8.18 μM. It is also a low micromolar inhibitor of SPAK T233E in vitro (IC50 = 13.03 μM)[1].
Cell Research Cell lines HEK293 cells
Concentrations 1, 3, 10, 20, 50 μM
Incubation Time 30 min
Method

HEK293 cells were first treated with Rafoxanide or Closantel at the indicated concentrations or STOCK1S5 0699 (10 μM) for 30 min and then either left untreated or treated with hypotonic buffer for 30 min to activate WNK-SPAK/OSR1 signalling. The cells were then harvested, and the lysates were probed for phospho-NKCC1 T203, T207 and T212, total NKCC1, phospho-SPAKS373, total SPAK and GAPDH as a loading control.

Chemical Information & Solubility

Molecular Weight 626.01 Formula

C19H11Cl2I2NO3
CAS No. 22662-39-1 SDF --
Smiles C1=CC(=CC=C1OC2=C(C=C(C=C2)NC(=O)C3=C(C(=CC(=C3)I)I)O)Cl)Cl
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (159.74 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


Molecular Weight Calculator

In vivo
Batch:

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In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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