Home DNA Damage/DNA Repair G-quadruplex modulator Pyridostatin Trifluoroacetate Salt For research use only.

Pyridostatin Trifluoroacetate Salt

Synonyms: RR82 Trifluoroacetate Salt

Pyridostatin Trifluoroacetate Salt is a G-quadruplexe stabilizer with Kd of 490 nM in a cell-free assay, which targets a series of proto-oncogenes.

Pyridostatin Trifluoroacetate Salt Chemical Structure

Pyridostatin Trifluoroacetate Salt Chemical Structure

CAS No. 1472611-44-1

Purity & Quality Control

Pyridostatin Trifluoroacetate Salt Related Products

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MRC5 Cytotoxicity assay 72 hrs Cytotoxicity against human MRC5 cells assessed as cell growth inhibition after 72 hrs by MTT assay, IC50=5.38μM. 29323491
KB-3-1 qHTS assay P-glycoprotein substrates identified in KB-3-1 adenocarcinoma cell line, qHTS therapeutic library screen 31515284
Click to View More Cell Line Experimental Data

Biological Activity

Description Pyridostatin Trifluoroacetate Salt is a G-quadruplexe stabilizer with Kd of 490 nM in a cell-free assay, which targets a series of proto-oncogenes.
Targets
G-quadruplexe [1]
(Cell-free assay)
490 nM(Kd)
In vitro
In vitro

Pyridostatin decreases the proliferation of MRC-5–SV40 cells and various cancer cell lines, and induces cell-cycle arrest by DNA-damage checkpoint activation. Pyridostatin also reduces SRC-dependent cell motility in MDA-MB-231 cells by interacting with G-quadruplex motifs in SRC. [2]

Pyridostatin decreases EBNA1 synthesis via inhibition of G-quadruplexes. [3]
Cell Research Cell lines MRC-5–SV40 cells and various cancer cell lines
Concentrations 10 μM
Incubation Time 48 h
Method

Cells are plated at equal confluence and are left either untreated or were treated with 2 μM pyridostatin continually during the indicated time before harvesting the cells. Cells from individual plates are trypsinized and counted in a Coulter counter. Graphs represent the total cell numbers at each time interval, and the error bars represent the s.e.m. Data represent three independent experiments.
In Vivo
In vivo
Animal Research Animal Models CB17‐SCID mice
Dosages 7.5 mg/kg
Administration i.v.

Chemical Information & Solubility

Molecular Weight 710.7 Formula

C31H32N8O5·C2HF3O2
CAS No. 1472611-44-1 SDF Download Pyridostatin Trifluoroacetate Salt SDF
Smiles C1=CC=C2C(=C1)C(=CC(=N2)NC(=O)C3=CC(=CC(=N3)C(=O)NC4=NC5=CC=CC=C5C(=C4)OCCN)OCCN)OCCN.C(=O)(C(F)(F)F)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (140.7 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 30 mg/mL

Ethanol : 9 mg/mL


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In vivo
Batch:

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In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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