Pyridostatin Trifluoroacetate Salt

Catalog No.S7444 Batch:S744402

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Technical Data

Formula

C31H32N8O5·C2HF3O2
Molecular Weight 710.7 CAS No. 1472611-44-1
Solubility (25°C)* In vitro DMSO 100 mg/mL (140.7 mM)
Water 100 mg/mL (140.7 mM)
Ethanol 13 mg/mL (18.29 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Pyridostatin Trifluoroacetate Salt is a G-quadruplexe stabilizer with Kd of 490 nM in a cell-free assay, which targets a series of proto-oncogenes.
Targets
G-quadruplexe [1]
(Cell-free assay)
490 nM(Kd)
In vitro

Pyridostatin decreases the proliferation of MRC-5–SV40 cells and various cancer cell lines, and induces cell-cycle arrest by DNA-damage checkpoint activation. Pyridostatin also reduces SRC-dependent cell motility in MDA-MB-231 cells by interacting with G-quadruplex motifs in SRC. [2]

Pyridostatin decreases EBNA1 synthesis via inhibition of G-quadruplexes. [3]
In vivo

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    MRC-5–SV40 cells and various cancer cell lines

  • Concentrations

    10 μM

  • Incubation Time

    48 h

  • Method

    Cells are plated at equal confluence and are left either untreated or were treated with 2 μM pyridostatin continually during the indicated time before harvesting the cells. Cells from individual plates are trypsinized and counted in a Coulter counter. Graphs represent the total cell numbers at each time interval, and the error bars represent the s.e.m. Data represent three independent experiments.
Animal Study:

[4]
  • Animal Models

    CB17‐SCID mice

  • Dosages

    7.5 mg/kg

  • Administration

    i.v.

Selleck's Pyridostatin Trifluoroacetate Salt has been cited by 12 publications

Polyubiquitinated PCNA triggers SLX4-mediated break-induced replication in alternative lengthening of telomeres (ALT) cancer cells [ Nucleic Acids Res, 2024, gkae785] PubMed: 39291733
BRCA1 and 53BP1 regulate reprogramming efficiency by mediating DNA repair pathway choice at replication-associated double-strand breaks [ Cell Rep, 2024, 43(4):114006] PubMed: 38554279
Investigating G-quadruplex structures in RPGR gene: Implications for understanding X-linked retinal degeneration [ Heliyon, 2024, 10(8):e29828] PubMed: 38699732
RAD54L2-mediated DNA damage avoidance pathway specifically preserves genome integrity in response to topoisomerase 2 poisons [ Sci Adv, 2023, 9(49):eadi6681] PubMed: 38055811
G-quadruplex binders as cytostatic modulators of innate immune genes in cancer cells [ Nucleic Acids Res, 2021, gkab500] PubMed: 34139015
Differential responses of neurons, astrocytes, and microglia to G-quadruplex stabilization [ Aging (Albany NY), 2021, 13(12):15917-15941] PubMed: 34139671
Characterization of an active LINE-1 in the naked mole-rat genome [ Sci Rep, 2021, 11(1):5725] PubMed: 33707548
hnRNP H/F Drive RNA G-quadruplex-mediated Translation Linked to Genomic Instability and Therapy Resistance in Glioblastoma [ Nat Commun, 2020, 27;11(1):2661] PubMed: 32461552
Replication Stress Induces ATR/CHK1-Dependent Nonrandom Segregation of Damaged Chromosomes. [ Mol Cell, 2020, 22 pii: S1097-2765(20)30231-8] PubMed: 32353258
Regulation of Autophagy by DNA G-quadruplexes [ Autophagy, 2020, 18] PubMed: 32420812

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.