Home Ubiquitin p97 inhibitor NPD8733 For research use only.

NPD8733

NPD8733 is an inhibitor of cancer cell-enhanced fibroblast migration that specifically binds to valosin-containing protein (VCP)/p97, a member of the ATPase-associated with diverse cellular activities (AAA+) protein family.

NPD8733 Chemical Structure

NPD8733 Chemical Structure

CAS No. 696655-62-6

Purity & Quality Control

Batch: S445301 DMSO]31 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 98.90%
98.90

NPD8733 Related Products

Signaling Pathway

p97 Signaling Pathways

p97 Signaling Pathway

Biological Activity

Description NPD8733 is an inhibitor of cancer cell-enhanced fibroblast migration that specifically binds to valosin-containing protein (VCP)/p97, a member of the ATPase-associated with diverse cellular activities (AAA+) protein family.
Targets
p97 [1]
In vitro
In vitro

NPD8733 is identified as inhibiting the enhanced migration of fibroblasts when co-cultured with cancer cells, in which valosin-containing protein (VCP)/p97 is the target protein for NPD8733 .[2]
Cell Research Cell lines NIH3T3 cells and MCF7 cells
Concentrations 30 μl
Incubation Time 3 h
Method

NPD8733 beads were prepared. The co-cultured NIH3T3 cells and MCF7 cells (5:1; 2 × 105 and 0.4 × 105, respectively) were collected, washed with PBS, and resuspended in binding buffer and a protease inhibitor mixture tablet. After cells were lysed by homogenization with sonication, the insoluble material was discarded by centrifugation, and the supernatant was collected as the cell lysate. Eight milligrams of protein from the cell lysate was incubated with 30 μl of control, NPD8733 beads for 3 h at 4 °C using a rotating mixer.

Chemical Information & Solubility

Molecular Weight 309.32 Formula
C18H15NO4
CAS No. 696655-62-6 SDF --
Smiles COC1=CC=C(C=C1)C(=O)C2=C(NC(C)=O)C3=CC=CC=C3O2
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 31 mg/mL ( (100.21 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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