NPD8733

Catalog No.S4453 Batch:S445301

Technical Data

Formula	C₁₈H₁₅NO₄
Molecular Weight	309.32	CAS No.	696655-62-6
Solubility (25°C)*	In vitro	DMSO	31 mg/mL (100.21 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

NPD8733 is an inhibitor of cancer cell-enhanced fibroblast migration that specifically binds to valosin-containing protein (VCP)/p97, a member of the ATPase-associated with diverse cellular activities (AAA+) protein family.

Targets

p97 ^[1]

In vitro

NPD8733 is identified as inhibiting the enhanced migration of fibroblasts when co-cultured with cancer cells, in which valosin-containing protein (VCP)/p97 is the target protein for NPD8733 .^[2]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines NIH3T3 cells and MCF7 cells Concentrations 30 μl Incubation Time 3 h Method NPD8733 beads were prepared. The co-cultured NIH3T3 cells and MCF7 cells (5:1; 2 × 10⁵ and 0.4 × 10⁵, respectively) were collected, washed with PBS, and resuspended in binding buffer and a protease inhibitor mixture tablet. After cells were lysed by homogenization with sonication, the insoluble material was discarded by centrifugation, and the supernatant was collected as the cell lysate. Eight milligrams of protein from the cell lysate was incubated with 30 μl of control, NPD8733 beads for 3 h at 4 °C using a rotating mixer.

Cell Assay:^{^[2]}

Cell lines
NIH3T3 cells and MCF7 cells
Concentrations
30 μl
Incubation Time
3 h
Method
NPD8733 beads were prepared. The co-cultured NIH3T3 cells and MCF7 cells (5:1; 2 × 10⁵ and 0.4 × 10⁵, respectively) were collected, washed with PBS, and resuspended in binding buffer and a protease inhibitor mixture tablet. After cells were lysed by homogenization with sonication, the insoluble material was discarded by centrifugation, and the supernatant was collected as the cell lysate. Eight milligrams of protein from the cell lysate was incubated with 30 μl of control, NPD8733 beads for 3 h at 4 °C using a rotating mixer.

References

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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