Home Metabolism IDO/TDO inhibitor IDO-IN-2 For research use only.

IDO-IN-2

Synonyms: RG 6078, NLG-919 analogue, GDC-0919 analogue

IDO-IN-2 (RG 6078, NLG-919 analogue, GDC-0919 analogue) is a a potent IDO1 inhibitor with IC50 of 38 nM.

IDO-IN-2 Chemical Structure

IDO-IN-2 Chemical Structure

CAS No. 1402836-58-1

Purity & Quality Control

IDO-IN-2 Related Products

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa Function assay Inhibition of IDO1 in IFNgamma-stimulated human HeLa cells incubated for 24 hrs, EC50=0.061 μM 26642377
HeLa Function assay Inhibition of IDO1 in IFN-gamma stimulated human HeLa cells assessed as inhibition of kynurenine production pre-incubated for 1 hr followed by IFN-gamma addition and measured after 18 hrs, IC50=0.004μM. 29398543
Click to View More Cell Line Experimental Data

Biological Activity

Description IDO-IN-2 (RG 6078, NLG-919 analogue, GDC-0919 analogue) is a a potent IDO1 inhibitor with IC50 of 38 nM.
Targets
IDO1 [2]
(Cell-free assay)
38 nM
In vitro
In vitro

IDO-IN-2 (NLG-919 analogue, Compound 24) contains an imidazoleisoindole core and exhibits potent inhibitory activity to IDO1 with the IC50 value of 38 nM. IDO-IN-2 inhibits IDO activity with EC50 of 61 nM in HeLa cellular assay. The binding mode of IDO-IN-2 to IDO1 is experimentally available and shows a direct coordinative interaction to the sixth coordination site of ferric heme.[2] IDO-IN-2 (Compound 7) has been used as reference compound in other studies to validate high-throughput screening assay for IDO1 inhibition and develop immunostimulatory nanomicellar carrier.[1]
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02471846 Completed
Solid Tumor
Genentech Inc.
 July 28 2015 Phase 1

Chemical Information & Solubility

Molecular Weight 282.38 Formula

C18H22N2O
CAS No. 1402836-58-1 SDF Download IDO-IN-2 SDF
Smiles C1CCC(CC1)C(CC2C3=CC=CC=C3C4=CN=CN24)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 56 mg/mL ( (198.31 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 56 mg/mL

Water : Insoluble


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In vivo
Batch:

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In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
NLG919 has two chiral centers in compound, so which isoform (RR, RS,SR,SS) of NLG919 that you sale ? or it

Answer:
NLG919 is synthesized as a racemate, a mixture of four isoform (RR, RS,SR,SS).

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