IDO-IN-2

Catalog No.S7111 Batch:S711105

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Technical Data

Formula

C18H22N2O
Molecular Weight 282.38 CAS No. 1402836-58-1
Solubility (25°C)* In vitro DMSO 56 mg/mL (198.31 mM)
Ethanol 56 mg/mL (198.31 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
2.8mg/ml Taking the 1 mL working solution as an example, add 50 μL of 56 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 95% Corn oil
2.8mg/ml Taking the 1 mL working solution as an example, add 50 μL of 56 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description IDO-IN-2 (RG 6078, NLG-919 analogue, GDC-0919 analogue) is a a potent IDO1 inhibitor with IC50 of 38 nM.
Targets
IDO1 [2]
(Cell-free assay)
38 nM
In vitro

IDO-IN-2 (NLG-919 analogue, Compound 24) contains an imidazoleisoindole core and exhibits potent inhibitory activity to IDO1 with the IC50 value of 38 nM. IDO-IN-2 inhibits IDO activity with EC50 of 61 nM in HeLa cellular assay. The binding mode of IDO-IN-2 to IDO1 is experimentally available and shows a direct coordinative interaction to the sixth coordination site of ferric heme.[2] IDO-IN-2 (Compound 7) has been used as reference compound in other studies to validate high-throughput screening assay for IDO1 inhibition and develop immunostimulatory nanomicellar carrier.[1]

Protocol (from reference)

Customer Product Validation

Data from [J Biomol Screen, 2014, 10.1177/1087057114536616]

Data from [Data independently produced by , , Biochem Pharmacol, 2018, 158:286-297]

Selleck's IDO-IN-2 has been cited by 15 publications

Bispecific prodrug nanoparticles circumventing multiple immune resistance mechanisms for promoting cancer immunotherapy [ Acta Pharm Sin B, 2022, 12(6):2695-2709] PubMed: 35755274
Cascade Immune Activation of Self-Delivery Biomedicine for Photodynamic Immunotherapy Against Metastatic Tumor [ Small, 2022, e2205694] PubMed: 36366925
Downregulation of miR-26 promotes invasion and metastasis via targeting interleukin-22 in cutaneous T-cell lymphoma [ Int J Mol Sci, 2022, 23(7)3981] PubMed: 35409342
Tryptophan potentiates CD8+ T cells against cancer cells by TRIP12 tryptophanylation and surface PD-1 downregulation [ J Immunother Cancer, 2021, 9(7)e002840] PubMed: 34326168
Decreased IDO1 Dependent Tryptophan Metabolism in Aged Lung during Influenza [ Eur Respir J, 2020, 2000443] PubMed: 33243840
Ultrasound-Driven Biomimetic Nanosystem Suppresses Tumor Growth and Metastasis through Sonodynamic Therapy, CO Therapy, and Indoleamine 2,3-Dioxygenase Inhibition [ ACS Nano, 2020, 14(7):8985-8999] PubMed: 32662971
Discovery of Novel Indoleamine 2,3-Dioxygenase 1 (IDO1) and Histone Deacetylase 1 (HDAC1) Dual Inhibitors Derived from the Natural Product Saprorthoquinone [ Molecules, 2020, 25(19)E4494] PubMed: 33007982
Discovery of Novel Indoleamine 2,3-Dioxygenase 1 (IDO1) and Histone Deacetylase 1 (HDAC1) Dual Inhibitors Derived from the Natural Product Saprorthoquinone [ Molecules, 2020, 25(19)E4494] PubMed: 33007982
Inhibition of the BET family reduces its new target gene IDO1 expression and the production of L-kynurenine. [ Cell Death Dis, 2019, 10(8):557] PubMed: 31324754
Displacement Induced Off-On Fluorescent Biosensor Targeting IDO1 Activity in Live Cells. [ Anal Chem, 2019, 91(23):14943-14950] PubMed: 31714063

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.