Home Ubiquitin DUB inhibitor MF-094 For research use only.

MF-094

MF-094, a potent and selective USP30 inhibitor (IC50=0.12 μM), accelerates diabetic wound healing by inhibiting the NLRP3 inflammasome.

MF-094 Chemical Structure

MF-094 Chemical Structure

CAS No. 2241025-68-1

Purity & Quality Control

Batch: E109801 DMSO]100 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.49%
99.49

MF-094 Related Products

Signaling Pathway

DUB Signaling Pathways

DUB Signaling Pathway

Biological Activity

Description MF-094, a potent and selective USP30 inhibitor (IC50=0.12 μM), accelerates diabetic wound healing by inhibiting the NLRP3 inflammasome.
Targets
USP30 [1]
0.12 μM
In vitro
In vitro

MF-094 restores viability and migration of AGE-treated HSF2 cells. MF-094 treatment decreases protein levels of NLRP3 and its downstream target caspase-1 p20.[2]
Cell Research Cell lines HSF2 human skin fibroblasts
Concentrations 100 nM
Incubation Time 24 h
Method

HSF2 cells are treated with USP30 inhibitor MF-094 (100 nM) or transduced with shUSP30 lentivirus vector for 24 h, following 200 μg/ml AGE treatment for 24 h. After treatment, HSF2 cells (5 × 105 cells/well) are plated in 96- well plates and cultured for 0, 12, 24, and 48 h. Then, 10 ml CCK-8 solution is added to cells for cell viability assay. The protein levels of NLRP3 and P20 are determined by Western blot.
In Vivo
In vivo

MF-094 treatment facilitates wound healing in diabetic rats.[2]
Animal Research Animal Models Streptozotocin (STZ) induced diabetic rats
Dosages 50 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 535.70 Formula

C30H37N3O4S
CAS No. 2241025-68-1 SDF --
Smiles CC(C)(C)N[S](=O)(=O)C1=CC=CC2=C1C=CC=C2NC(=O)C(CC3=CC=CC=C3)NC(=O)C4CCCCC4
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 100 mg/mL ( (186.67 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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