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LC3-mHTT-IN-AN1

LC3-mHTT-IN-AN1 is a mHTT-LC3 linker compound that interacts with both mutant huntingtin protein (mHTT) and LC3B. LC3-mHTT-IN-AN1 reduces the levels of mHTT in an allele-selective manner in cultured Huntington disease (HD) mouse neurons.

LC3-mHTT-IN-AN1 Chemical Structure

LC3-mHTT-IN-AN1 Chemical Structure

CAS No. 486443-73-6

Purity & Quality Control

Batch: S077801 DMSO]82 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.85%
99.85

LC3-mHTT-IN-AN1 Related Products

Biological Activity

Description LC3-mHTT-IN-AN1 is a mHTT-LC3 linker compound that interacts with both mutant huntingtin protein (mHTT) and LC3B. LC3-mHTT-IN-AN1 reduces the levels of mHTT in an allele-selective manner in cultured Huntington disease (HD) mouse neurons.
Targets
LC3 [1] mHTT [1]
In vitro
In vitro

LC3-mHTT-IN-AN1 reduces the levels of mHTT in an allele-selective manner in cultured HD mouse neurons.[1]
Cell Research Cell lines mouse primary cultured neurons and iPS-cell-derived neurons
Concentrations 10, 50, 100, and 300 nM
Incubation Time --
Method

LC3-mHTT-IN-AN1 was diluted in culture medium to 10×concentrations and added to the plated cells: for primary cultured neurons and iPS-cell-derived neurons, LC3-mHTT-IN-AN1 was added 5 days after plating; The cells were then collected 2 days later for measurement of HTT levels. For detection of HTT-LC3 colocalization, the cells were fixed 4 h after compound treatment. For caspase-3 activation detection, the cells were stressed (BDNF removal for iPS-cell-derived neurons) 1 day after compound treatment, and tested at the indicated time points.

Chemical Information & Solubility

Molecular Weight 411.04 Formula

C15H9Br2NO3
CAS No. 486443-73-6 SDF --
Smiles C1=CC2=C(C=C1Br)C(=CC3=CC(=C(C(=C3)Br)O)O)C(=O)N2
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 82 mg/mL ( (199.49 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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