LC3-mHTT-IN-AN1

Catalog No.S0778 Batch:S077801

Technical Data

Formula	C₁₅H₉Br₂NO₃
Molecular Weight	411.04	CAS No.	486443-73-6
Solubility (25°C)*	In vitro	DMSO	82 mg/mL (199.49 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

LC3-mHTT-IN-AN1 is a mHTT-LC3 linker compound that interacts with both mutant huntingtin protein (mHTT) and LC3B. LC3-mHTT-IN-AN1 reduces the levels of mHTT in an allele-selective manner in cultured Huntington disease (HD) mouse neurons.

Targets

LC3 ^[1]	mHTT ^[1]

In vitro

LC3-mHTT-IN-AN1 reduces the levels of mHTT in an allele-selective manner in cultured HD mouse neurons.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines mouse primary cultured neurons and iPS-cell-derived neurons Concentrations 10, 50, 100, and 300 nM Incubation Time -- Method LC3-mHTT-IN-AN1 was diluted in culture medium to 10×concentrations and added to the plated cells: for primary cultured neurons and iPS-cell-derived neurons, LC3-mHTT-IN-AN1 was added 5 days after plating; The cells were then collected 2 days later for measurement of HTT levels. For detection of HTT-LC3 colocalization, the cells were fixed 4 h after compound treatment. For caspase-3 activation detection, the cells were stressed (BDNF removal for iPS-cell-derived neurons) 1 day after compound treatment, and tested at the indicated time points.

Cell Assay:^{^[1]}

Cell lines
mouse primary cultured neurons and iPS-cell-derived neurons
Concentrations
10, 50, 100, and 300 nM
Incubation Time
--
Method
LC3-mHTT-IN-AN1 was diluted in culture medium to 10×concentrations and added to the plated cells: for primary cultured neurons and iPS-cell-derived neurons, LC3-mHTT-IN-AN1 was added 5 days after plating; The cells were then collected 2 days later for measurement of HTT levels. For detection of HTT-LC3 colocalization, the cells were fixed 4 h after compound treatment. For caspase-3 activation detection, the cells were stressed (BDNF removal for iPS-cell-derived neurons) 1 day after compound treatment, and tested at the indicated time points.

References

[1] Zhaoyang Li, et al. Nature. 2019 Nov;575(7781):203-209.

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