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L-Kynurenine

Name: L-Kynurenine
Brand: Selleck Chemicals
SKU: S5839
Rating: 5 (1 reviews)

Synonyms: (S)-Kynurenine

Catalog No.S5839 Purity:99.93%

L-Kynurenine ((S)-Kynurenine) is a aryl hydrocarbon receptor agonist. L-Kynurenine is a metabolite of the amino acid L-tryptophan used in the production of niacin and a central compound of the tryptophan metabolism pathway.

L-Kynurenine Chemical Structure

CAS No. 2922-83-0

Purity & Quality Control

Batch: Purity: 99.93%

99.93

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Biological Activity

Description	L-Kynurenine ((S)-Kynurenine) is a aryl hydrocarbon receptor agonist. L-Kynurenine is a metabolite of the amino acid L-tryptophan used in the production of niacin and a central compound of the tryptophan metabolism pathway.

In vitro
In vitro	Kynurenine (Kyn), the tryptophan (Trp) metabolite and an AHR ligand in hepatocytes, accumulates to cellular levels to cause an elevated rate of AHR activation, and stimulates AHR transcriptional activity quickly. An excess production of Kyn via increased cellular IDO1 levels and activity leads to amplified AHR signaling ultimately giving rise to obesity.^[1]
Cell Research	Cell lines	H1L7.5c3 mouse hepatocytes
	Concentrations	0.1, 1, 10, 100 μM
	Incubation Time	24 h
	Method	H1L7.5c3 cells are seeded in white-walled, white-bottomed 96-well plates at 4000 cells/well and incubated for 24 hr in culture medium. After the 24-hr incubation, the medium is removed, and the cells are washed once with Dulbecco's Phosphate Buffered Saline. Cells are treated with Kynurenine (Kyn) for an additional 24 hr, all of which are dissolved in DMSO unless otherwise noted. Then, cells are removed from incubation and allowed to equilibrate to room temperature for 15min. After equilibration, the medium is removed and the cells are washed twice with at room temperature with DPBS. The cells are lysed with 20 μl/well 1 Passive Lysis Buffer and shaken for 20 min at room temperature. Luciferase activity is recorded programmed to inject 50 μl Luciferase Assay Reagent per well with a 10 sec integration of emitted luminescence.

In Vivo
In vivo	L-kynurenine shows a dose-dependent significant neuroprotective effect before the hypoxia-ischemia of rats, and blocks the induction of c-fos immunoreactivity in cerebral cortex following ischemia.^[2]
Animal Research	Animal Models	7-day-old rats
	Dosages	100, 200, or 300 mg/kg; 50, 100, 200, 300, 400 mg/kg
	Administration	i.p.

References

Chemical Information & Solubility

Molecular Weight	208.21	Formula	C₁₀H₁₂N₂O₃
CAS No.	2922-83-0	SDF	--
Smiles	C1=CC=C(C(=C1)C(=O)CC(C(=O)O)N)N
Storage (From the date of receipt)	3 years -20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C powder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 21 mg/mL ( (100.85 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 14 mg/mL

Ethanol : Insoluble

Molecular Weight Calculator

In vivo Batch: Add solvents to the product individually and in order.					In vivo Formulation Calculator
	Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	1.05mg/ml (5.04mM)	Taking the 1 mL working solution as an example, add 50 μL of 21 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.	In vivo Formulation Calculator
Clear solution	5% DMSO 1 95% Corn oil	1.05mg/ml (5.04mM)	Taking the 1 mL working solution as an example, add 50 μL of 21 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	saline	10.0mg/ml (48.03mM)	Taking the 1 mL working solution as an example, add 10 mg of this product to 1 ml of physiological saline (0.9% NaCL solution), mix evenly to make it clear, The mixed solution should be used immediately for optimal results.

Preparing Stock Solutions

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
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Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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