Home Metabolism AhR agonist L-Kynurenine For research use only.

L-Kynurenine

Synonyms: (S)-Kynurenine

L-Kynurenine ((S)-Kynurenine) is a aryl hydrocarbon receptor agonist. L-Kynurenine is a metabolite of the amino acid L-tryptophan used in the production of niacin and a central compound of the tryptophan metabolism pathway.

L-Kynurenine Chemical Structure

L-Kynurenine Chemical Structure

CAS No. 2922-83-0

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L-Kynurenine Related Products

Biological Activity

Description L-Kynurenine ((S)-Kynurenine) is a aryl hydrocarbon receptor agonist. L-Kynurenine is a metabolite of the amino acid L-tryptophan used in the production of niacin and a central compound of the tryptophan metabolism pathway.
In vitro
In vitro

Kynurenine (Kyn), the tryptophan (Trp) metabolite and an AHR ligand in hepatocytes, accumulates to cellular levels to cause an elevated rate of AHR activation, and stimulates AHR transcriptional activity quickly. An excess production of Kyn via increased cellular IDO1 levels and activity leads to amplified AHR signaling ultimately giving rise to obesity.[1]
Cell Research Cell lines H1L7.5c3 mouse hepatocytes
Concentrations 0.1, 1, 10, 100 μM
Incubation Time 24 h
Method

H1L7.5c3 cells are seeded in white-walled, white-bottomed 96-well plates at 4000 cells/well and incubated for 24 hr in culture medium. After the 24-hr incubation, the medium is removed, and the cells are washed once with Dulbecco's Phosphate Buffered Saline. Cells are treated with Kynurenine (Kyn) for an additional 24 hr, all of which are dissolved in DMSO unless otherwise noted. Then, cells are removed from incubation and allowed to equilibrate to room temperature for 15min. After equilibration, the medium is removed and the cells are washed twice with at room temperature with DPBS. The cells are lysed with 20 μl/well 1 Passive Lysis Buffer and shaken for 20 min at room temperature. Luciferase activity is recorded programmed to inject 50 μl Luciferase Assay Reagent per well with a 10 sec integration of emitted luminescence.
In Vivo
In vivo

L-kynurenine shows a dose-dependent significant neuroprotective effect before the hypoxia-ischemia of rats, and blocks the induction of c-fos immunoreactivity in cerebral cortex following ischemia.[2]
Animal Research Animal Models 7-day-old rats
Dosages 100, 200, or 300 mg/kg; 50, 100, 200, 300, 400 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 208.21 Formula

C10H12N2O3
CAS No. 2922-83-0 SDF --
Smiles C1=CC=C(C(=C1)C(=O)CC(C(=O)O)N)N
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 21 mg/mL ( (100.85 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 14 mg/mL

Ethanol : Insoluble


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