Home Metabolism AhR agonist FICZ For research use only.

FICZ

Synonyms: 6-Formylindolo[3,2-b]carbazole

FICZ (6-Formylindolo[3,2-b]carbazole) is a potent agonist of aryl hydrocarbon receptor (AhR) that is efficiently metabolized by AHR-regulated cytochrome P4501 enzymes.

FICZ Chemical Structure

FICZ Chemical Structure

CAS No. 172922-91-7

Purity & Quality Control

Batch: S968201 DMSO]57 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.05%
99.05

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Biological Activity

Description FICZ (6-Formylindolo[3,2-b]carbazole) is a potent agonist of aryl hydrocarbon receptor (AhR) that is efficiently metabolized by AHR-regulated cytochrome P4501 enzymes.
Targets
AhR [1]
In vitro
In vitro

In vitro studies in cells show that CYP1 inhibition in the presence of FICZ results in enhanced AHR activation, suggesting that FICZ accumulates in the cell when its metabolism is blocked.[1] FICZ significantly upregulates the gene expression of filaggrin in both HaCaT cells and NHEKs in an AHR-dependent manner, but does not affect the gene expression of other barrier-related proteins.[2]
Cell Research Cell lines Human immortalized HaCaT cells, normal human epidermal keratinocytes (NHEKs)
Concentrations 1 nM, 10 nM, 100 nM
Incubation Time 1 h, 3 h, 5 h
Method

HaCaTcells or NHEKs (1×104 cells/well) are platedon an 8-well μ-slide for 48 h, and treated with or without FICZ for 3 or 1 h, respectively. Then, the cells are washed with phosphate-buffered saline (PBS), fixed with acetone for 10 min, and blocked with 5% (w/v) bovine serum albumin in PBS for 30 min. The cells are then incubated with primary rabbit anti-AHR antibody (1:100) overnight at 4 ℃. Specific binding is detected using a horseradish peroxidase-conjugated goat anti-rabbit anti-body followed by tyramide labeling with green-fluorescent Alexa Fluor 488 for 1 h at room temperature in accordance with the manufacturer's instructions.
In Vivo
In vivo

FICZ improves the atopic dermatitis-like skin inflammation, clinical scores, and transepidermal water loss in NC/Nga mice compared with those of control mice. On histology, FICZ significantly reduces the epidermal and dermal thickness as well as the number of mast cells. Topical FICZ also significantly reduces the gene expression of Il22.[2]
Animal Research Animal Models 9–10 week-old female NC/Nga mice
Dosages 100 mg/affected area
Administration Topical

Chemical Information & Solubility

Molecular Weight 284.31 Formula

C19H12N2O
CAS No. 172922-91-7 SDF --
Smiles O=CC1=C2C(=CC3=C1[NH]C4=C3C=CC=C4)[NH]C5=C2C=CC=C5
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 57 mg/mL ( (200.48 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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