Home Immunology & Inflammation IRAK inhibitor JH-X-119-01 For research use only.

JH-X-119-01

JH-X-119-01 is a highly potent and selective covalent inhibitor of IRAK1 with IC50 of 9 nM.

JH-X-119-01 Chemical Structure

JH-X-119-01 Chemical Structure

CAS No. 2227368-54-7

Purity & Quality Control

Batch: S978001 DMSO]90 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 98.8%
98.8

JH-X-119-01 Related Products

Biological Activity

Description JH-X-119-01 is a highly potent and selective covalent inhibitor of IRAK1 with IC50 of 9 nM.
Targets
IRAK1 [1]
(Cell-free assay)
9 nM
In vitro
In vitro

JH-X-119-01 is a highly efficient and selective covalent inhibitor of interleukin 1 (IL-1) receptor-associated kinase (IRAK) 1 and irreversibly labels IRAK1 at C302, showing moderate cell killing effects with EC50s ranging from 0.59 to 9.72 μM in a panel of WM cells, DLBCL cells, and lymphoma cells.<sup><a class="sref" href="#s_ref">[1]</a></sup>
Cell Research Cell lines WM cells, DLBCL cells, and lymphoma cells
Concentrations --
Incubation Time 72 h
Method

CellTiter-Glo assay

Chemical Information & Solubility

Molecular Weight 452.46 Formula

C25H20N6O3
CAS No. 2227368-54-7 SDF --
Smiles C=CC(=O)NC1=CC=CC(=C1)C(=O)NC2=CC=C(NC(=O)C3=NC(=CC=C3)C4=CC=N[NH]4)C=C2
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 90 mg/mL ( (198.91 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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