JH-X-119-01

Catalog No.S9780 Batch:S978001

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Technical Data

Formula

C25H20N6O3
Molecular Weight 452.46 CAS No. 2227368-54-7
Solubility (25°C)* In vitro DMSO 90 mg/mL (198.91 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5% DMSO 95% Corn oil
2.25mg/ml Taking the 1 mL working solution as an example, add 50 μL of 45 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
4.5mg/ml Taking the 1 mL working solution as an example, add 50 μL of 90 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description JH-X-119-01 is a highly potent and selective covalent inhibitor of IRAK1 with IC50 of 9 nM.
Targets
IRAK1 [1]
(Cell-free assay)
9 nM
In vitro

JH-X-119-01 is a highly efficient and selective covalent inhibitor of interleukin 1 (IL-1) receptor-associated kinase (IRAK) 1 and irreversibly labels IRAK1 at C302, showing moderate cell killing effects with EC50s ranging from 0.59 to 9.72 μM in a panel of WM cells, DLBCL cells, and lymphoma cells.<sup><a class="sref" href="#s_ref">[1]</a></sup>

Protocol (from reference)

Cell Assay:

<sup><a class="sref" href="#s_ref">[1]</a></sup>
  • Cell lines

    WM cells, DLBCL cells, and lymphoma cells

  • Concentrations

    --

  • Incubation Time

    72 h

  • Method

    CellTiter-Glo assay

Selleck's JH-X-119-01 has been cited by 1 publication

IRAK1 inhibition blocks the HIV-1 RNA mediated pro-inflammatory cytokine response from microglia [ J Gen Virol, 2023, 104(5)001858] PubMed: 37256770

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.