Home Neuronal Signaling AChR inhibitor Jatrorrhizine For research use only.

Jatrorrhizine

Synonyms: Neprotin, Yatrorizine

Jatrorrhizine (Neprotin, Yatrorizine), one of the active constituents of Coptis chinensis Franch, has multiple bioactivities, such as hypoglycemic, antimicrobial, and antioxidant activities. It is an inhibitor of AChE with IC50 of 872 nM and demonstrates >115-fold selectivity for AChE over BuChE.

Jatrorrhizine Chemical Structure

Jatrorrhizine Chemical Structure

CAS No. 3621-38-3

Purity & Quality Control

Batch: S388401 DMSO]67 mg/mL]false]]]false]]]false Purity: 99.37%
99.37

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Signaling Pathway

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Biological Activity

Description Jatrorrhizine (Neprotin, Yatrorizine), one of the active constituents of Coptis chinensis Franch, has multiple bioactivities, such as hypoglycemic, antimicrobial, and antioxidant activities. It is an inhibitor of AChE with IC50 of 872 nM and demonstrates >115-fold selectivity for AChE over BuChE.
Targets
AChE [1]
(Cell-free assay)
872 nM
In vitro
In vitro Jatrorrhizine hydrochloride suppresses C8161 cell proliferation in a dose-dependent manner, with a half-maximal inhibitory concentration of 47.4±1.6 μmol/l. however, it does not induce significant cellular apoptosis at doses up to 320 μmol/l. Mechanistic studies show that JH-induced C8161 cell cycle arrest at the G0/G1 transition, which is accompanied by overexpression of the cell cycle-suppressive genes p21 and p27 at higher doses[2].
Cell Research Cell lines Human melanoma C8161 cells
Concentrations 0, 80, 160, 320 μmol/L
Incubation Time 48 h
Method C8161 cells are grown in T75 flasks and treated with the indicated JH for 48 h. After the treatment, the cells are fixed with 70% ethanol overnight at 41℃, washed with PBS, and resuspended in 500 ml PBS with 100 mg/ml RNase. After incubation for 30 min at 37℃, 2.5 ml (10 mg/ml) PI solution is added and incubated on ice for another 30 min in the dark. The DNA contents of PI-stained cells are analyzed using flow cytometry.
In Vivo
In vivo JH reduces metastatic melanoma cell-mediated neovascularization[2]. JH ameliorates hyperlipidemia via the suppression of lipogenesis and the enhancement of lipid oxidation in the liver[3].
Animal Research Animal Models 7-week-old male C57BL/6 mice
Dosages 20 and 100 mg/kg
Administration intragastric injections

Chemical Information & Solubility

Molecular Weight 338.38 Formula

C20H20NO4
CAS No. 3621-38-3 SDF Download Jatrorrhizine SDF
Smiles COC1=C(C2=C[N+]3=C(C=C2C=C1)C4=CC(=C(C=C4CC3)O)OC)OC
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 67 mg/mL ( (198.0 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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