Home Immunology & Inflammation MIF inhibitor ISO-1 For research use only.

ISO-1

Synonyms: MIF Antagonist

ISO-1 (MIF Antagonist) is an inhibitor of MIF (Macrophage migration inhibitory factor) D-dopachrome tautomerase activity with an IC50 of ~7 μM.

ISO-1 Chemical Structure

ISO-1 Chemical Structure

CAS No. 478336-92-4

Purity & Quality Control

ISO-1 Related Products

Biological Activity

Description ISO-1 (MIF Antagonist) is an inhibitor of MIF (Macrophage migration inhibitory factor) D-dopachrome tautomerase activity with an IC50 of ~7 μM.
Targets
MIF [1]
7 μM
In vitro
In vitro

ISO-1 significantly inhibits the cytokine activity in vitro. It inhibits tumor necrosis factor release from macrophages isolated from LPStreated wild type mice but has no effect on cytokine release from MIF-deficient macrophages[2].
Cell Research Cell lines RAW 267.4 macrophages
Concentrations 1-100 μM
Incubation Time 30 min
Method

RAW 267.4 macrophages were treated with various concentrations of ISO-1 (1-100 μM) 30 min prior to LPS addition. Macrophages were collected 2 h after activation with LPS and washed one time in PBS (pH 7.4). Nuclear extract was isolated using NE-PER Nuclear and Cytoplasmic Extraction Reagents.
In Vivo
In vivo

ISO-1 significantly increases the survival rate in severe sepsis induced by endotoxin or cecal ligation and puncture (CLP)[2]. It could prevent airway hyperresponsiveness (AHR) in mouse ovalbumin (OVA)-challenge models. ISO-1 attenuated ozone-induced cell recruitment, cytokine release and AHR in the mouse but did not affect measures of emphysema[3]. ISO-1 protects photoreceptors and reduces gliosis in experimental retinal detachment[4].
Animal Research Animal Models Male Balb/C mice, ∼8 weeks old, subjectd to endotoxemia or cecal ligation and puncture (CLP)
Dosages 3.5-35 mg/kg
Administration i.p.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02284919 Active not recruiting
Breast Cancer|Breast Neoplasm
University of Pennsylvania
 November 2014 Phase 1
NCT02204202 Terminated
Lung Disease
Washington University School of Medicine|National Heart Lung and Blood Institute (NHLBI)
 February 2014 --

Chemical Information & Solubility

Molecular Weight 235.24 Formula

C12H13NO4
CAS No. 478336-92-4 SDF --
Smiles COC(=O)CC1CC(=NO1)C2=CC=C(C=C2)O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 47 mg/mL ( (199.79 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 47 mg/mL

Water : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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