ISO-1

Catalog No.S7732 Batch:S773203

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Technical Data

Formula

C12H13NO4
Molecular Weight 235.24 CAS No. 478336-92-4
Solubility (25°C)* In vitro DMSO 47 mg/mL (199.79 mM)
Ethanol 47 mg/mL (199.79 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description ISO-1 (MIF Antagonist) is an inhibitor of MIF (Macrophage migration inhibitory factor) D-dopachrome tautomerase activity with an IC50 of ~7 μM.
Targets
MIF [1]
7 μM
In vitro

ISO-1 significantly inhibits the cytokine activity in vitro. It inhibits tumor necrosis factor release from macrophages isolated from LPStreated wild type mice but has no effect on cytokine release from MIF-deficient macrophages[2].
In vivo

ISO-1 significantly increases the survival rate in severe sepsis induced by endotoxin or cecal ligation and puncture (CLP)[2]. It could prevent airway hyperresponsiveness (AHR) in mouse ovalbumin (OVA)-challenge models. ISO-1 attenuated ozone-induced cell recruitment, cytokine release and AHR in the mouse but did not affect measures of emphysema[3]. ISO-1 protects photoreceptors and reduces gliosis in experimental retinal detachment[4].

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    RAW 267.4 macrophages

  • Concentrations

    1-100 μM

  • Incubation Time

    30 min

  • Method

    RAW 267.4 macrophages were treated with various concentrations of ISO-1 (1-100 μM) 30 min prior to LPS addition. Macrophages were collected 2 h after activation with LPS and washed one time in PBS (pH 7.4). Nuclear extract was isolated using NE-PER Nuclear and Cytoplasmic Extraction Reagents.
Animal Study:

[2]
  • Animal Models

    Male Balb/C mice, ∼8 weeks old, subjectd to endotoxemia or cecal ligation and puncture (CLP)

  • Dosages

    3.5-35 mg/kg

  • Administration

    i.p.

Selleck's ISO-1 has been cited by 17 publications

Pericyte signaling via soluble guanylate cyclase shapes the vascular niche and microenvironment of tumors [ EMBO J, 2024, 43(8):1519-1544] PubMed: 38528180
MicroRNA-451 Regulates Angiogenesis in Intracerebral Hemorrhage by Targeting Macrophage Migration Inhibitory Factor [ Mol Neurobiol, 2024, 10.1007/s12035-024-04207-3] PubMed: 38743209
Moxibustion of Zusanli (ST36) and Shenshu (BL23) alleviates the inflammation of rheumatoid arthritis in rats through regulating macrophage migration inhibitory factor/glucocorticoids signaling [ J Tradit Chin Med, 2024, 44(2):353-361] PubMed: 38504541
MIF is a critical regulator of mononuclear phagocytic infiltration in hepatocellular carcinoma [ iScience, 2023, 26(8):107273] PubMed: 37520719
MIF Increases sFLT1 Expression in Early Uncomplicated Pregnancy and Preeclampsia [ Int J Mol Sci, 2023, 24(12)10050] PubMed: 37373198
Therapeutic targeting of tumor spheroids in a 3D microphysiological renal cell carcinoma-on-a-chip system [ Neoplasia, 2023, 10.1016/j.neo.2023.100948] PubMed: 37944353
Ischemic stroke protected by ISO-1 inhibition of apoptosis via mitochondrial pathway [ Sci Rep, 2023, 13(1):2788] PubMed: 36797398
On the origin of metastases: Induction of pro-metastatic states after impending cell death via ER stress, reprogramming, and a cytokine storm [ Cell Rep, 2022, 38(10):110490] PubMed: 35263600
Exosomes from adipose-derived stem cells regulate M1/M2 macrophage phenotypic polarization to promote bone healing via miR-451a/MIF [ Stem Cell Res Ther, 2022, 13(1):149] PubMed: 35395782
Downregulation of macrophage migration inhibitory factor attenuates NLRP3 inflammasome mediated pyroptosis in sepsis-induced AKI [ Cell Death Discov, 2022, 8(1):61] PubMed: 35165294

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.